Automated multi-step purification system

Patent metadata

Field	Value
Publication number	US-10429359-B2
Application number	US-201515116940-A
Country	US
Kind code	B2
Filing date	Feb 13, 2015
Priority date	Feb 14, 2014
Publication date	Oct 1, 2019
Grant date	Oct 1, 2019

Abstract

Automated two step chromatography purification system comprising a, system controller, a capture flow path comprising at least one pump, an elution flow path comprising at least one pump, and a valve arrangement for selective connection of two capture columns to the capture flow path and the elution flow path respectively such that both flow paths may be operated simultaneously and in parallel.

First claim

The invention claimed is:

1. An automated multi-step chromatography purification system arranged to perform a plurality of isolated multi-step purification cycles for purifying target molecules from separate feed sources, comprising:
a system controller comprising a memory storing instructions for controlling components of the system,
two or more purification sections, each representing a purification step with one of the purification sections being a capture section comprising a first capture column and a second capture column and another one of the purification sections being a subsequent purification section comprising a purification column,
at least one pump for driving the purification,
a valve arrangement for controlling the fluid flow in the system, wherein the valve arrangement comprises a source valve connected to the at least one pump,
a capture flow path, a capture column eluent flow path, and a purification column eluent flow path all share the same source valve,
wherein: the capture columns and the source valve is arranged to simultaneously
(i) connecting, through the capture flow path the second capture column to one of the separate feed sources for capturing the respective target molecule, and
(ii) eluting, through the capture column eluent flow path a captured target molecule from the first capture column to the purification column and for preparing the eluted first capture column for a subsequent capture phase,
wherein the subsequent purification section comprises a smaller number of purification column eluent flow paths compared to the number of capture columns, each purification column eluent flow path comprising a purification column, whereby eluent flow from each of the capture column is separately purified in said purification column eluent flow path with intermediate cleaning of the purification column eluent flow path to keep subsequent purifications isolated, and
wherein each purification section being in direct fluidic communication with the subsequent purification section without an intermediate sample storage component.

2. The system according to claim 1 wherein the capture column is an affinity chromatography column.

3. The system according to claim 1 wherein the subsequent purification section comprises one or more gel filtration columns.

4. The system according to claim 1 further comprising a feed filter section arranged in between the feed sources and the capture section, the feed filter section being arranged to selectively introduce a clean filter for each feed flow.

5. The system according to claim 1 further comprising an elute conditioning feed source arranged to provide a conditioning flow to the eluent flow in order to change one or more buffer parameters of the elution flow to a condition suitable for a subsequent purification section.

6. The system according to claim 1 further comprising an eluent filter section arranged in between the elute conditioning feed source and the subsequent purification section, the eluent filter section being arranged to selectively introduce a clean filter for each elution flow.

7. The system according to claim 1 further comprising one or more sensors connected to the system controller for providing input of the status of the purification process, the sensors being selected from the group of; pH sensor, conductivity sensor, UV absorption sensor, air sensor.

8. The system according to claim 1 being arranged to prepare the capture columns for a subsequent capture phase by a cleaning in place process and equilibration process to keep subsequent purifications isolated.

9. The system according to claim 1 wherein the intermediate cleaning of the eluent purification flow paths comprises a cleaning in place process and equilibration process.

10. An automated multi-step chromatography purification method comprising a plurality of isolated multi-step purification cycles for purifying target molecules from separate feed sources, using an automated chromatography system that comprises:
a system controller comprising a memory storing instructions for controlling the components of the system,
two or more purification sections, each representing a purification step and being in direct fluidic communication with the subsequent purification step without any intermediate sample storage component, comprising:
i. a capture section comprising a first capture column and a second capture column, and
ii. a subsequent purification sections that is subsequent to the capture section and comprises a smaller number of purification column eluent flow paths compared to the number of capture columns, each purification column eluent flow paths comprising a purification column,
at least one pump for driving the purification, and
a valve arrangement for controlling the fluid flow in the system, wherein the valve arrangement comprises a source valve connected to the at least one pump,
a capture flow path, a capture column eluent flow path, and a purification column eluent flow path all share the same source valve,
the method comprising:
(i) capturing, through the capture flow path, a target molecule onto the second capture column, and
(ii) simultaneously eluting, through the capture column eluent flow path, a captured target molecule from the first capture column, and preparing the eluted first capture column for a subsequent capture phase,
separately purifying each eluent flow from the capture section in one of said purification column eluent flow paths, and
intermediate cleaning each purification column eluent flow path before each subsequent purification to keep the target molecules isolated.

11. The method according to claim 10 wherein the capture column is an affinity chromatography column.

12. The method according to claim 10 wherein one of the purification sections comprises one or more gel filtration columns.

13. The method according to claim 10 further comprising a filtration step using a feed filter section arranged in between the feed sources and the capture section, the feed filter section being arranged to selectively introduce a clean filter for each feed flow.

14. The method according to claim 10 further comprising a step of using an elute conditioning feed source arranged to provide a conditioning flow to the eluent flow in order to change one or more buffer parameters of the elution flow to a condition suitable for a subsequent purification section.

15. The method according to claim 10 further comprising a filtration step using an eluent filter section arranged in between the elute conditioning feed source and the subsequent purification section, the eluent filter section being arranged to selectively introduce a clean filter for each elution flow.

16. The method according to claim 10 wherein the chromatography system further comprises one or more sensors connected to the system controller for providing input of the status of the purification process, the sensors being selected from the group of: pH sensor, conductivity sensor, UV absorption sensor, air sensor.

17. The method according to claim 10 wherein the step of preparing the capture columns for a subsequent capture phase comprises implementing a cleaning in place process and equilibration process to keep subsequent purifications isolated.

18. The method according to claim 10 wherein the intermediate cleaning of the eluent purification flow paths comprises a cleaning in place process and equilibration process.

19. An automated multi-step chromatography purification system arranged to perform a plural

Assignees

• Ge Healthcare Bio Sciences Ab

Inventors

• Eriksson Christer Olof
• Norrman Nils

Classifications

• G01N30/04 Primary

  Preparation or injection of sample to be analysed · CPC title

• B01D15/203

  Equilibration or regeneration · CPC title

• G01N30/468

  involving switching between different column configurations · CPC title

• B01D15/125

  Pre-filtration · CPC title

• B01D15/1885

  placed in parallel · CPC title