MEMS-based isothermal titration calorimetry

Patent metadata

Field	Value
Publication number	US-10429328-B2
Application number	US-201514603848-A
Country	US
Kind code	B2
Filing date	Jan 23, 2015
Priority date	Jul 24, 2012
Publication date	Oct 1, 2019
Grant date	Oct 1, 2019

Abstract

A microelectromechanical systems-based calorimetric device includes first and second micromixers and first and second thermally-isolated microchambers. A first solution including a sample and a reagent is introduced to the first microchamber via the first micromixer, and a second solution including a sample and a buffer is introduced to the second microchamber via the second micromixer. A thermopile measures the differential temperature between the first microchamber and the second microchamber and outputs a voltage representative of the difference. The output voltage can be used to calculate reaction parameters.

First claim

We claim:

1. A microelectromechanical systems-based calorimetric device for characterization of biomolecular interactions comprising:
a first micromixer;
a second micromixer;
a thermally-isolated reaction chamber in fluid contact with the first micromixer;
a thermally-isolated reference chamber in fluid contact with the second micromixer; and
a thermoelectric sensor configured to measure at least one temperature metric associated with the reaction chamber and the reference chamber;
wherein the first micromixer comprises a passive chaotic micromixer.

2. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the passive chaotic micromixer comprises a serpentine channel.

3. The microelectromechanical systems-based calorimetric device of claim 2 , wherein the serpentine channel comprises herringbone shaped ridges.

4. The microelectromechanical systems-based calorimetric device of claim 1 , further comprising a first inlet and a second inlet in fluid contact with the first micromixer.

5. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reaction chamber comprises a polydimethylsiloxane microchamber.

6. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reference chamber comprises a polydimethylsiloxane microchamber.

7. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reaction chamber comprises a serpentine chamber.

8. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reference chamber comprises a serpentine chamber.

9. The microelectromechanical systems-based calorimetric device of claim 1 further comprising a polyimide diaphragm that serves as a base for the reaction chamber.

10. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the thermoelectric sensor comprises a thermopile.

11. The microelectromechanical systems-based calorimetric device of claim 10 , wherein the thermopile comprises an antimony-bismuth thermopile.

12. The microelectromechanical systems-based calorimetric device of claim 10 , wherein a first thermopile junction is located on a first side of the reaction chamber.

13. The microelectromechanical systems-based calorimetric device of claim 12 , wherein a second thermopile junction is located on the first side of the reference chamber.

14. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reaction chamber is surrounded by an air cavity.

15. The microelectromechanical systems-based calorimetric device of claim 14 , wherein the air cavity comprises a serpentine channel.

16. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reference chamber is surrounded by an air cavity.

17. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the reaction chamber comprises a chamber temperature sensor.

18. The microelectromechanical systems-based calorimetric device of claim 17 , wherein the reaction chamber further comprises a heater.

19. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the at least one temperature metric comprises a differential temperature between the reaction chamber and the reference chamber.

20. The microelectromechanical systems-based calorimetric device of claim 1 , wherein the at least one temperature metric comprises a temperature of the reaction chamber and a temperature of the reference chamber.

Assignees

• Univ Columbia

Inventors

• Lin Qiao
• Wang Bin

Classifications

• B01L2300/0883

  Serpentine channels · CPC title

• G01K17/00

  Measuring quantity of heat (measuring temperature by calorimetry G01K3/00 - G01K11/00; specially adapted for measuring thermal properties of materials, e.g. specific heat, heat of combustion G01N) · CPC title

• B01L2300/1827

  using resistive heater · CPC title

• G01N25/482 Primary

  concerning the temperature responsive elements (measuring temperature or quantity of heat, thermally-sensitive elements G01K; thermoelectric devices H10N10/00, H10N15/00) · CPC title

• B01L2300/0877

  Flow chambers · CPC title