P-coumaroyl-CoA:monolignol transferase

Patent metadata

Field	Value
Publication number	US-10428342-B2
Application number	US-201214365744-A
Country	US
Kind code	B2
Filing date	Dec 14, 2012
Priority date	Dec 16, 2011
Publication date	Oct 1, 2019
Grant date	Oct 1, 2019

Abstract

The invention relates to nucleic acids encoding a p-coumaroyl-CoA:monolignol transferase and to inhibitory nucleic acids adapted to inhibit the expression and/or translation of a p-coumaroyl-CoA:monolignol transferase RNA. Inhibition of p-coumaroyl-CoA:monolignol transferase in plants improves the incorporation of monolignol ferulates into the lignin of plants, giving rise to plant biomass that is more easily processed into useful products such as paper and biofuels.

First claim

What is claimed:

1. A genetically modified grass plant comprising a feruloyl-CoA: monolignol transferase nucleic acid that encodes a polypeptide with at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 9, 20 and 21 operably linked to a heterologous promoter functional in cells of the plant, which plant has a knockdown or knockout of the grass plant's endogenous p-coumaroyl-CoA: monolignol transferase gene comprising a sequence with at least 95% sequence identity to SEQ ID NO:16 or 18.

2. The genetically modified plant of claim 1 , wherein the knockdown or knockout is a mutation selected from the group consisting of a point mutation, a deletion, a missense mutation, insertion or a nonsense mutation in the endogenous p-coumaroyl-CoA:monolignol transferase gene.

3. The genetically modified plant of claim 1 , wherein the knockdown or knockout comprises a point mutation, a deletion, a missense mutation, insertion or a nonsense mutation in the endogenous p-coumaroyl-CoA: monolignol transferase gene encoding a polypeptide with at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 17 or 24.

4. The genetically modified plant of claim 1 , wherein the knockdown or knockout comprises expression of at least one inhibitory nucleic acid comprising 20 nucleotides with at least 95% sequence identity to either strand of SEQ ID NO:16 or 18 nucleic acid sequence.

5. The genetically modified plant of claim 1 , wherein the knockdown or knockout reduces acylation of monolignols with p-coumarate.

6. The genetically modified plant of claim 1 , wherein the knockdown or knockout reduces acylation of monolignols with p-coumarate, where the monolignols are selected from the group consisting of p-coumaryl alcohol, coniferyl alcohol and sinapyl alcohol.

7. The genetically modified plant of claim 1 , wherein the knockdown or knockout reduces acylation of monolignols with p-coumarate by at least 30%.

8. The genetically modified plant of claim 1 , wherein the a feruloyl-CoA:monolignol transferase nucleic acid is operably linked to a promoter selected from the group consisting of a poplar xylem-specific secondary cell wall specific cellulose synthase 8 promoter, a cauliflower mosaic virus promoter, a Z10 promoter from a gene encoding a 10 kD zein protein, a Z27 promoter from a gene encoding a 27 kD zein protein, a pea rbcS gene promoter, or an actin promoter from rice.

9. The genetically modified plant of claim 1 , wherein the plant is selected from the species consisting of Miscanthus giganteus, Panicum virgatum (switchgrass), Zea mays (corn), Oryza sativa (rice), Saccharum sp. (sugar cane), Triticum sp. (wheat), Avena sativa (oats), Pennisetum glaucum (pearl millet), Setaria italica (foxtail millet), Sorghum sp., Bambuseae species (bamboo), Sorghastrum nutans (indiangrass), Tripsacum dactyloides (eastern gamagrass), Andropogon gerardii (big bluestem), Schizachyrium scoparium (little bluestem), Bouteloua curtipendula (sideoats grama), Silphium terebinthinaceum (prairie rosinweed), Pseudoroegneria spicata (bluebunch wheatgrass) Sorghum bicolor (sorghum) and Bachypodium distachyon (purple false brome).

10. The genetically modified plant of claim 1 , wherein the plant is fertile.

11. A seed from the genetically modified plant of claim 1 , wherein the seed comprises an expression cassette encoding an inhibitory nucleic acid that reduces expression of the plant's endogenous p-coumaroyl-CoA:monolignol transferase gene; or the seed comprises a mutation in the plant's endogenous p-coumaroyl-CoA:monolignoltransferase gene.

Assignees

• Wisconsin Alumni Res Found
• Univ Michigan State
• The Board Of Trustees Of Illinois State Univ

Inventors

• Wilkerson Curtis
• Ralph John
• Withers Saunia
• Sedbrook John

Classifications

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12N15/8255 Primary

  involving lignin biosynthesis · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title

• C12N15/1137

  against enzymes (viral enzymes C12N15/1131; receptors C12N15/1138) · CPC title