Encapsulated diagnostics and therapeutics in nanoparticles—conjugated to tropic cells and methods for their use

Patent metadata

Field	Value
Publication number	US-10426801-B2
Application number	US-201414256777-A
Country	US
Kind code	B2
Filing date	Apr 18, 2014
Priority date	Oct 18, 2011
Publication date	Oct 1, 2019
Grant date	Oct 1, 2019

Abstract

A therapeutic or diagnostic delivery vehicle is provided. The delivery vehicle may include one or more particles, such as microparticles, nanoparticles and stimuli-responsive particles, conjugated to a tropic cell that targets at least one pathological entity or site. In addition, a pharmaceutical composition is provided. The pharmaceutical composition may include, among other things, a particle conjugated to a tropic cell such as those discussed above and at least one diagnostic or therapeutic agent, such as those described herein. In some aspects, the tropic cell may target at least one pathological entity or site. Further, methods for diagnosing, monitoring or treating a pathological condition in a subject are provided. Such methods may include administering a therapeutically effective amount of the pharmaceutical composition to a subject.

First claim

What is claimed is:

1. A therapeutic delivery vehicle comprising a gold particle coated with a cationic thiol moiety internalized by a genetically modified neural stem cell, wherein the cationic thiol moiety is 11-mercaptoundecyltrimethylammonium bromide.

2. The therapeutic delivery vehicle of claim 1 , wherein the genetically modified neural stem cell is an immortalized neural stem cell.

3. The therapeutic delivery vehicle of claim 1 , wherein the genetically modified neural stem cell expresses cytosine deaminase, v-myc, or both cytosine deaminase and v-myc.

4. The therapeutic delivery vehicle of claim 1 , wherein the gold particle is a gold nanorod.

5. The therapeutic delivery vehicle of claim 1 , wherein the average longest dimension of the gold particle is about 41 nm.

6. The therapeutic delivery vehicle of claim 1 , wherein the gold particle is about 10 nm by about 41 nm.

Assignees

• Hope City

Inventors

• Aboody Karen
• Annala Alexander
• Mooney Rachael
• Berlin Jacob
• Weng Yiming

Classifications

• A61K49/085

  conjugated systems · CPC title

• A61K47/6929

  the form being a nanoparticle, e.g. an immuno-nanoparticle · CPC title

• A61K47/6901

  Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors · CPC title

• A61K35/30 Primary

  Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue · CPC title

• A61K47/6923

  the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb · CPC title