Detection of viable pathogens in analyte using culture chamber with magnetostrictive sensors

Patent metadata

Field	Value
Publication number	US-10422770-B2
Application number	US-201715631150-A
Country	US
Kind code	B2
Filing date	Jun 23, 2017
Priority date	Jun 23, 2016
Publication date	Sep 24, 2019
Grant date	Sep 24, 2019

Abstract

In at least one illustrative embodiment, a system may include a basin that includes an index plate positioned at a bottom of the basin. The basin is configured to receive a liquid analyte, such as a liquid food product or a nutrient broth. The index plate includes an array of multiple wells. Each well opens into an interior of the basin and is sized to receive a magnetostrictive sensor in a predetermined orientation. One or more sensor coils is positionable beneath each well. The basin may be filled with liquid analyte and magnetostrictive sensors may be positioned in the wells. The liquid analyte may be allowed to incubate at a controlled temperature. A controller may position a sensor coil beneath a well, apply a varying magnetic field to a magnetostrictive sensor in the well, and detect a frequency response of the magnetostrictive sensor. Other embodiments are described and claimed.

First claim

The invention claimed is:

1. A method for microorganism detection, the method comprising:
filling a basin with a liquid analyte, wherein the basin includes a index plate having an array of wells, wherein each well contains a magnetostrictive sensor that comprises magnetostrictive material, and wherein each well aligns the corresponding magnetostrictive sensor in a predetermined orientation;
incubating the liquid analyte at a controlled temperature in response to filling the basin;
positioning a sensor coil beneath a first well of the index plate;
applying a varying magnetic field, using the sensor coil, to a first magnetostrictive sensor within the first well in response to incubating the liquid analyte, wherein the first magnetostrictive sensor comprises a biorecognition element to bind with a microorganism;
detecting a frequency response of the first magnetostrictive sensor using the sensor coil while applying the varying magnetic field; and
determining whether a microorganism is present based on the detected frequency response.

2. The method of claim 1 , wherein positioning the sensor coil beneath the first well comprises translating the basin to position the sensor coil beneath the first well.

3. The method of claim 1 , wherein incubating the liquid analyte comprises incubating the liquid analyte at the controlled temperature for about 4 to 6 hours.

4. The method of claim 1 , further comprising:
positioning the sensor coil beneath a second well of the index plate in response to detecting the frequency response of the first magnetostrictive sensor;
applying a varying magnetic field, using the sensor coil, to a second magnetostrictive sensor within the second well; and
detecting a frequency response of the second magnetostrictive sensor using the sensor coil while applying the varying magnetic field.

5. The method of claim 1 , wherein filling the basin with the liquid analyte comprises flowing the liquid analyte continuously through the basin.

6. The method of claim 1 , wherein the analyte comprises a liquid food product.

7. The method of claim 1 , wherein the analyte comprises a liquid growth medium.

8. The method of claim 1 , wherein determining whether a microorganism is present based on the detected frequency response comprises comparing the frequency response of the first magnetostrictive sensor to a frequency response of a control magnetostrictive sensor, wherein the control magnetostrictive sensor does not include a biorecognition element to bind with a microorganism.

9. The method of claim 1 , further comprising:
detecting an initial frequency response of the first magnetostrictive sensor using the sensor coil while applying the varying magnetic field, in response to filling the basin with the liquid analyte and prior to incubating the liquid analyte;
wherein incubating the liquid analyte comprises incubating the liquid analyte in response to detecting the initial frequency response; and
wherein determining whether a microorganism is present comprises comparing the frequency response of the first magnetostrictive sensor to the initial frequency response.

10. The method of claim 1 , wherein incubating the liquid analyte at the controlled temperature comprises heating the liquid analyte with an infrared heating element.

Assignees

• Univ Auburn

Inventors

• Chin Bryan A
• Horikawa Shin
• Cheng Zhongyang

Classifications

• G01N2291/106

  one or more transducer arrays · CPC title

• G01N2291/0255

  (Bio)chemical reactions, e.g. on biosensors · CPC title

• G01N29/222

  Constructional or flow details for analysing fluids (optoacoustic fluid cells G01N29/2425) · CPC title

• G01N33/569

  for microorganisms, e.g. protozoa, bacteria, viruses · CPC title

• G01N33/54326

  Magnetic particles · CPC title