Centrifugal separation method

Patent metadata

Field	Value
Publication number	US-10420876-B2
Application number	US-201615269237-A
Country	US
Kind code	B2
Filing date	Sep 19, 2016
Priority date	Mar 29, 2013
Publication date	Sep 24, 2019
Grant date	Sep 24, 2019

Abstract

A storage portion forming a storage space 10, includes an inclined inner wall portion 20 that is connected to a base portion so that the diameter of the inclined inner wall portion gradually decreases; a concave portion 22 is formed at a part of the inclined inner wall portion; and the concave portion 22 includes a concave portion side surface 22 b that is connected to a concave portion bottom surface 22 a. The concave portion 22 is formed at a position, where the concave portion crosses an interface S between the specimen centrifuged during rotation and air, in a radial direction with respect to the central axis; and the maximum width of the concave portion 22 in a circumferential direction around the central axis is included in a range of 2 mm to a length of 20% of the whole circumference.

First claim

What is claimed is:

1. A centrifugal separation method, using a centrifugal separation container, which centrifugally separating a first specific gravity component and a second specific gravity component, of which a specific gravity of the second gravity component is higher than a specific gravity of the first specific gravity component, contained in a specimen injected into a storage space by rotating a container about a central axis of the container as a rotation axis, the centrifugal separation method comprising:
injecting a specimen into a storage portion that forms the storage space in the centrifugal separation container,
wherein the storage portion includes an inclined inner wall portion of which a diameter gradually decreases from an upper end toward a lower end thereof and the lower end is connected to a base portion, and
a concave portion formed at a part of the inclined inner wall portion,
the concave portion includes a concave portion side surface that is connected to a concave portion bottom surface with a width of the concave portion gradually decreases toward the concave portion bottom surface from the inclined inner wall portion;
rotating the centrifugal separation container about the central axis of the container as a rotation axis,
wherein an interface between the specimen centrifuged during rotation and air, in a radial direction with respect to the central axis crosses where the concave portion is formed.

2. The centrifugal separation method according to claim 1 ,
wherein a connecting portion between the inclined inner wall portion and the concave portion side surface has a curvature.

3. The centrifugal separation method according to claim 1 ,
wherein a connecting portion between the concave portion side surface and the concave portion bottom surface has a curvature.

4. The centrifugal separation method according to claim 2 ,
wherein a connecting portion between the concave portion side surface and the concave portion bottom surface has a curvature.

5. The centrifugal separation method according to claim 1 ,
wherein a mean depth of the concave portion is 0.5 mm or more.

6. The centrifugal separation method according to claim 2 ,
wherein a mean depth of the concave portion is 0.5 mm or more.

7. The centrifugal separation method according to claim 1 ,
wherein the maximum depth of the concave portion is in the range of 0.5 mm to 2 mm.

8. The centrifugal separation method according to claim 1 ,
wherein the maximum width of the concave portion is 10 mm or less.

9. The centrifugal separation method according to claim 1 ,
wherein the concave portion has the shape of a fan.

10. The centrifugal separation method according to claim 1 ,
wherein one concave portion is formed.

11. The centrifugal separation method according to claim 10 , further comprising:
a balancing portion that offsets a deviation of the center of gravity from the central axis caused by the formation of the concave portion to balance the container.

12. The centrifugal separation method according to claim 11 ,
wherein the balancing portion is provided on the inclined inner wall portion at a position that is symmetrical to the position of the concave portion with respect to the central axis.

13. The centrifugal separation method according to claim 1 ,
wherein two to four concave portions are formed.

14. The centrifugal separation method according to claim 13 ,
wherein the concave portions are evenly disposed in the circumferential direction.

15. The centrifugal separation method according to claim 1 ,
wherein the maximum length of the concave portion is in the range of 5 mm to 15 mm.

16. The centrifugal separation method according to claim 1 ,
wherein the concave portion is formed from the uppermost portion of the inclined inner wall portion.

17. The centrifugal separation method according to claim 1 ,
wherein the storage portion includes a trap portion that is connected to an upper end of the inclined inner wall portion and forms a trap space housing the second specific gravity component when the specimen is centrifugally separated and a layer structure body is formed on an outer peripheral side of the storage space.

18. The centrifugal separation method according to claim 1 ,
wherein a thixotropic separating agent, which has a specific gravity between the specific gravity of the first specific gravity component and the specific gravity of the second specific gravity component, is provided in the storage space.

19. A centrifugal separation method comprising:
the centrifugal separation method according to claim 1 ; and
a step of holding the container by a container holder that rotates about a central axis of the container as a rotation axis.

Assignees

• Fujifilm Corp

Inventors

• Nishio Tomonori
• Nishijima Kazuteru

Classifications

• B04B11/04

  Periodical feeding or discharging; Control arrangements therefor · CPC title

• A61J1/05

  for collecting, storing or administering blood, plasma or medical fluids {(multiple bags systems for separating or storing blood components A61M1/0209); Infusion or perfusion containers} · CPC title

• B04B5/0407

  for liquids contained in receptacles (B04B5/0442 takes precedence) · CPC title

• B04B1/08

  of conical shape · CPC title

• B04B9/14

  Balancing rotary bowls (balancing per se G01M); {Schrappers} · CPC title