Cell carrier for skin tissue regeneration containing chitooligosaccharide and method for producing the same

Patent metadata

Field	Value
Publication number	US-10420858-B2
Application number	US-201615754508-A
Country	US
Kind code	B2
Filing date	Apr 6, 2016
Priority date	Oct 16, 2015
Publication date	Sep 24, 2019
Grant date	Sep 24, 2019

Abstract

Provided are a cell carrier for skin tissue regeneration using chitooligosaccharide and a method for producing the same. The cell carrier according to the present invention includes fish-derived collagen (FC), alginate (A), and chitooligosaccharide (COS1) of 1 to 3 kDa, and thus has excellent biocompatibility with dermal fibroblasts and has an excellent effect of being utilized as a cell carrier for skin tissue regeneration.

First claim

The invention claimed is:

1. A method of preparing a collagen-alginate-chitooligosaccharide cell carrier for skin tissue regeneration, the method comprising the steps of:
(a) dissolving fish-derived collagen and alginate in 0.02 M acetic acid to prepare collagen-alginate (FCA) solution in a weight ratio (w/w) of 70:30;
(b) freeze-drying the FCA solution obtained in step (a) to prepare a collagen-alginate (FCA) scaffold;
(c) immersing the FCA scaffold obtained in step (b) in a cross-linker solution for 3 to 5 hours to cross-link the same;
(d) adding 1 to 3 kDa chitooligosaccharide (COS1) in an amount of 0.5 to 5% (w/w) with respect to the total weight of the cross-linker solution and cross-linking the same for 20 to 30 hours while the FCA scaffold of step (c) is immersed in the cross-linker solution; and
(e) immersing the FCA/COS1 cell carrier obtained in step (d) in distilled water for 48 hours, washing the cell carrier, and then freeze-drying the cell carrier,
wherein the cross-linker in step (c) is one or more selected from the group consisting of N,N′-carbonyldiimidazole (CDI), N,N′-dicyclohexylcarbonylimide (DCC), N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), 4-(4,6-dimethoxy-1,3,5-triazine)-4-methylmorpholium (DMT-MM), 2-benzotriazole-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU), 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (HODhbt), benzotriazol-1-oxy-tris-pyrrolidone-phosphonium hexafluorophosphate (PyBOP), benzotriazol-1-yl-oxy-tris-dimethylamino-phosphonium hexafluorophosphate (BOP), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS).

2. A collagen-alginate-chitooligosaccharide cell carrier for skin tissue regeneration prepared according to the method of claim 1 .

Assignees

• Nat Univ Pukyong Ind Univ Coop Found

Inventors

• Jung Won-Kyo
• Ko Seok-Chun
• Oh Gun-Woo

Classifications

• A61L27/60

  Materials for use in artificial skin · CPC title

• A61L27/26

  Mixtures of macromolecular compounds · CPC title

• C12N11/06

  attached to the carrier via a bridging agent · CPC title

• A61L27/3691

  characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation · CPC title

• A61L2430/40

  Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking · CPC title