Scaffold for tissue growth and repair

Patent metadata

Field	Value
Publication number	US-10420856-B2
Application number	US-201615242711-A
Country	US
Kind code	B2
Filing date	Aug 22, 2016
Priority date	Mar 12, 2009
Publication date	Sep 24, 2019
Grant date	Sep 24, 2019

Abstract

Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.

First claim

We claim:

1. An electroactive structure for growing and differentiating a differentiable cell comprising a three dimensional matrix of electro spun fibers; wherein
the electro spun fibers are formed by electrospinning a biocompatible synthetic piezoelectric polymer at an electric potential of between about 15 kV and about 30 kV;
wherein the electro spun fibers comprise a higher β phase content than electro spun fibers formed by electrospinning the biocompatible synthetic piezoelectric polymer at an electric potential of less than about 15 kV;
wherein the electro spun fibers are annealed, wherein annealing of the electro spun fibers further increases the β phase content and enhances piezoelectric characteristics;
wherein the matrix of electro spun fibers forms a scaffold for supporting cell growth and differentiation; and
wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into a cell with either an osteogenic or chondrogenic phenotype.

2. The electroactive structure according to claim 1 , wherein the biocompatible synthetic piezoelectric polymer is a homopolymer, a copolymer or combination thereof.

3. The electroactive structure according to claim 2 , wherein the homopolymer is a polyvinylidene fluoride (PVDF) homopolymer or a trifluoroethylene homopolymer (TrFE).

4. The electroactive structure according to claim 2 , wherein the copolymer is a poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer.

5. The electroactive structure of claim 1 , wherein the matrix of electrospun fibers further comprises a growth factor capable of further promoting the differentiation of the mesenchymal stem cell into a cell with osteogenic or chondrogenic phenotype.

6. The electroactive structure of claim 5 , wherein the growth factor is associated with the matrix of electrospun fibers through at least one of a covalent interaction, a non-covalent interaction or a combination of both.

7. The electroactive structure according to claim 1 , wherein the matrix of electrospun fibers is forms a non-woven mesh of nanofibers, microfibers or a combination of both.

8. The electroactive structure according to claim 1 , wherein the electrospun fibers are arranged in the matrix randomly, are substantially aligned or are a combination of both.

9. The electroactive structure according to claim 1 , wherein the fibers are thermally or chemically annealed.

10. The electroactive structure according to claim 1 , wherein the osteogenic or chondrogenic phenotype is demonstrated by at least one of increased collagen expression, growth or a combination thereof.

11. The electro active structure according to claim 1 , wherein the fibers are formed by electrospinning a biocompatible synthetic piezoelectric polymer at an electric potential of about 25 kV.

12. The electro active structure according to claim 1 further comprising a mesenchymal stem cell.

13. The electro active structure according to claim 1 further comprising a cell with osteogenic or chondrogenic phenotype, wherein the cell has differentiated from a mesenchymal stem cell.

14. An electro active structure for growing and differentiating a differentiable cell comprising a three dimensional matrix of electro spun fibers; wherein
the electro spun fibers are formed by electrospinning a biocompatible synthetic piezoelectric polymer selected from the group consisting of a polyvinylidene fluoride (PVDF) homopolymer, a trifluoroethylene homopolymer (TrFE) and a poly(vinylidene fluoride trifluoroethylene) (PVDF-TrFE) copolymer at an electric potential of between about 15 kV and about 30 kV;
wherein the electro spun fibers comprise a higher β phase content than electro spun fibers formed by electrospinning the biocompatible synthetic piezoelectric polymer at an electric potential of less than about 15 kV;
wherein the electro spun fibers are annealed, wherein annealing of the electro spun fibers further increases the β phase content and enhances piezoelectric characteristics;
wherein the matrix of electro spun fibers forms a scaffold for supporting cell growth and differentiation; and
wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype.

Assignees

• New Jersey Inst Technology

Inventors

• Arinzeh Treena
• Collins George
• Lee Yee-Shuan

Classifications

• A61P19/08

  for bone diseases, e.g. rachitism, Paget's disease · CPC title

• A61P19/04

  for non-specific disorders of the connective tissue · CPC title

• C12N5/0618

  Cells of the nervous system · CPC title

• A61L2300/414

  Growth factors · CPC title

• A61L27/54

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title