Nucleic acids and methods for detecting pathogens and beneficial microorganisms

Patent metadata

Field	Value
Publication number	US-10415098-B2
Application number	US-201415032325-A
Country	US
Kind code	B2
Filing date	Nov 5, 2014
Priority date	Nov 6, 2013
Publication date	Sep 17, 2019
Grant date	Sep 17, 2019

Abstract

The present invention relates to nucleic acids and methods for detecting pathogens and beneficial microorganisms.

First claim

The invention claimed is:

1. A method for detecting by hybridization techniques at least one pathogen and/or one beneficial microorganism in a sample, said method using at least forty and up to one hundred different nucleic acids, each nucleic acid chosen from (i), (ii) and/or (iii) below:
(i) the sequences selected from the group consisting of:

GGGTTGCTTGCTTGCGAGCTCC;
(SEQ ID No 1)

CTGGTGTTTGGACTCGCCTTAAAAC;
(SEQ ID No 2)

GCACATATTTTGCGCTTTGTATCAGG;
(SEQ ID No 3)

TCCGCCAGGGAARACCAAAACTCT;
(SEQ ID No 4)

CGGAGCGCGGGCCGTCGCG;
(SEQ ID No 5)

ACTTATACCCAAAACGTTGCCTCG;
(SEQ ID No 6)

CATTATCGAGTTTACGCTCCATAAC;
(SEQ ID No 7)

GCGCAGCTATTAGATCTACGGTG;
(SEQ ID No 8)

GCAAGGCTGGAGTATTTTATTACCCT;
(SEQ ID No 9)

CCGTGGCCTTGTTGCCACGCCC;
(SEQ ID No 10)

ATTGGGGCCTTGTTGCCACACCC;
(SEQ ID No 11)

TTTTGCGCTTTGTCCAGTTGCGG;
(SEQ ID No 12)

GATTCGTCGCCCCCCCTCCTGG;
(SEQ ID No 13)

GACCTTATTCAAACCTTTTTTTCAGTT;
(SEQ ID No 14)

GCTGTTGGGGACCGGCTCACCCG;
(SEQ ID No 15)

TGACCGTTGTCACGAGACGACTTTAT;
(SEQ ID No 16)

CTTGGTGTTGGGAGCTGCAGTCC;
(SEQ ID No 17)

CCATTGCGTAGTAGTAAAACCC;
(SEQ ID No 18)

AACGCGCTTCGTTCGGAGGCTT;
(SEQ ID No 19)

TTCAACCCTCAAGCCCCCGGGTTTG;
(SEQ ID No 20)

CGAAGTAGTGATATTCCGCATCGG;
(SEQ ID No 21)

GTTAGGGGGTCCCCTCTCCGG;
(SEQ ID No 22)

CTCGGTCTCGAGCCGCCGG;
(SEQ ID No 23)

ACACCCCATTGAACCTATTTATTTTYAA;
(SEQ ID No 24)

ACATCTCGCGCTTTGCATTCAGAA;
(SEQ ID No 25)

CGATTTTGGGGGGTGGCTAGTGC;
(SEQ ID No 26)

CTCTGAGTACGAAAAGAACCTGAAA;
(SEQ ID No 27)

TGCTGGCTCTTCTAGAGTCGGCTC;
(SEQ ID No 28)

GGCCAGACGACAGCCATAAACC;
(SEQ ID No 29)

CGCCGGTGGACTACCTAAACTCT;
(SEQ ID No 30)

CGTCGTCGCTGTTCGCAAGGAC;
(SEQ ID No 31)

TCGGGCAACGGAACCAGGCGC;
(SEQ ID No 32)

GTTGCCTCGGCGGGCACGGC;
(SEQ ID No 33)

CCAGTTATATAGGCACCCAATAAGCC;
(SEQ ID No 34)

CACCAAACCAGCTTGGGAAACCTT;
(SEQ ID No 35)

GTCACGTGGTCTTGGTTTTGAA;
(SEQ ID No 36)

CGCAACCGGGAGCCGCGGCGCGG;
(SEQ ID No 37)

TGGACTGGCTTCGGCTAGACTGG;
(SEQ ID No 38)

ACCATAGCTCAGTTGCTTGGCTTTT;
(SEQ ID No 39)

TGGCGAATGTTTGGACTTCGGTCT;
(SEQ ID No 40)

GCGCAAGCTGGGGTGGRCGAG;
(SEQ ID No 41)

GAACTTGTGTCTCTGCGGCGCG;
(SEQ ID No 42)

AACTGGTGAACCGTAGCTGTGTGGT;
(SEQ ID No 43)

CCGTACATTAAACTTGACTTTCTTCC;
(SEQ ID No 44)

GGAACTCCACCCTTGAATACACTG;
(SEQ ID No 45)

CGACCCCTTTTATAATTCACCCAAC;
(SEQ ID No 46)

CTAAAAAACCCCTCATAACCTTTTTTT;
(SEQ ID No 47)

CTCCTAAAACCCAATATCTTATTTTTAAG;
(SEQ ID No 48)

CAATACTGCCATCTTGTTTTTGAAGG;
(SEQ ID No 49)

ATACTTGCCATCTTTTTGGAAGG;
(SEQ ID No 50)

CCTAAAAACCCCCCTTATCA;
(SEQ ID No 51)

CAACCTTTTTGGAGTATTCTAATGAT;
(SEQ ID No 52)

ATACTGCCATCTTATTKAAGGGAGAC;
(SEQ ID No 53)

CAGGGCTATCCCCCTGCCAGG;
(SEQ ID No 54)

GGAGGGTTGCGCACTTTGTGCGTG;
(SEQ ID No

Assignees

• Omya Int Ag

Inventors

• Di Maiuta Nicola
• Egli Konrad
• Glaubitz Joachim
• Urwyler Simon

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q1/689 Primary

  for bacteria · CPC title

• C12Q2600/16

  Primer sets for multiplex assays · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12Q2600/112

  Disease subtyping, staging or classification · CPC title