Methods, devices, and systems for microfluidic stress emulation

Patent metadata

Field	Value
Publication number	US-10413901-B2
Application number	US-201515507616-A
Country	US
Kind code	B2
Filing date	Aug 28, 2015
Priority date	Aug 29, 2014
Publication date	Sep 17, 2019
Grant date	Sep 17, 2019

Abstract

Provided herein is technology relating to microfluidic devices and particularly, but not exclusively, to devices, methods, systems, and kits for imparting stresses on a fluid flowing through a microfluidic device that is designed to mimic a stress profile of a macrofluidic device or pathology.

First claim

We claim:

1. A method for monitoring platelet activation state in a subject, the method comprising:
a) modeling the shear stress profile of a pathology in a medical device,
wherein the pathology is a cardiovascular pathology,
wherein the medical device is a mechanical circulatory support device,
wherein the modeling comprises one or more of device thrombogenicity emulation, producing a probability density function of the medical device, and determining individual particle trajectories for particles flowing through the medical device;

b) reproducing the shear stress profile of the pathology in the device in a microfluidic device;
c) flowing a sample from the subject through the microfluidic device, wherein the sample comprises platelets separated from a blood sampled obtained from the subject,
wherein the sample has a volume of 1 to 100 nanoliters, 1 to 1000 nanoliters, 1 to 100 microliters, or 1 to 1000 microliters; and

d) testing a platelet activation state of the sample.

2. The method of claim 1 wherein the pathology is a deviation from a normal geometry or flow characteristic.

3. The method of claim 1 wherein the pathology is an arterial narrowing, stenosis, outpouching, anurysm or pseudoaneurysm, dissection, flap, fistula, constriction, thrombus, intinal hyperplasia, or tumor.

4. The method of claim 1 wherein the medical device is a ventricular assist device, stent, total artificial heart, or valve.

5. The method of claim 1 further comprising adjusting the dose of a drug or other therapeutic agent administered to the subject and/or adjusting the dose of an anti-thrombosis drug administered to the subject.

6. The method of claim 1 wherein the sample is acquired from the patient 1 to 10 times a day.

Assignees

• Univ Arizona
• Univ New York State Res Found
• Milano Politecnico

Inventors

• Slepian Marvin J
• Redaelli Alberto C
• Rasponi Marco
• Bluestein Danny

Classifications

• G01N2800/224

  Haemostasis or coagulation · CPC title

• A61B5/14546

  for measuring analytes not otherwise provided for, e.g. ions, cytochromes · CPC title

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N2800/226

  Thrombotic disorders, i.e. thrombo-embolism irrespective of location/organ involved, e.g. renal vein thrombosis, venous thrombosis · CPC title