Method for handheld diagnostics and assays
US-10094820-B2 · Oct 9, 2018 · US
US10412958B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10412958-B2 |
| Application number | US-201415025571-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 17, 2014 |
| Priority date | Oct 29, 2013 |
| Publication date | Sep 17, 2019 |
| Grant date | Sep 17, 2019 |
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Provided is a device for vitrification-based cryopreservation which enables easy and reliable vitrification freezing of cells or tissues. The device of the present invention for cell or tissue cryopreservation by vitrification has a porous structure made of a material having a refractive index of 1.45 or less as a vitrification solution absorber.
Opening claim text (preview).
The invention claimed is: 1. A device for cryopreserving eggs or embryos by vitrification, the device comprising a vitrification solution absorber having a porous structure made of a material having a refractive index of 1.45 or less, wherein after a cell or tissue immersed in a vitrification solution is placed on said vitrification solution absorber, the vitrification solution absorber absorbs excess vitrification solution surrounding the cell or tissue, the diameter of the largest opening of the porous structure measured according to the method for bubble point testing specified in JIS K3832 is 0.75 μm or less, and a thickness of the porous structure is 25 to 150 μm. 2. The device according to claim 1 , wherein the porous structure is made of fluororesin. 3. The device according to claim 1 , wherein the diameter of the largest opening measured according to the method for bubble point testing specified in JIS K3832 of the porous structure is 0.02 to 0.75 μm. 4. The device according to claim 1 , wherein the diameter of the largest opening measured according to the method for bubble point testing specified in JIS K3832 of the porous structure is 0.2 to 0.75 μm. 5. The device according to claim 2 , wherein the diameter of the largest opening measured according to the method for bubble point testing specified in JIS K3832 of the porous structure is 0.02 to 0.75 μm. 6. The device according to claim 2 , wherein the diameter of the largest opening measured according to the method for bubble point testing specified in JIS K3832 of the porous structure is 0.2 to 0.75 μm.
Means for packing or storing viable microorganisms (casings for storing cell samples A61B10/0096, preservation of living parts of the human or animal body A01N1/10) · CPC title
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Temperature processes, e.g. following predefined temperature changes over time · CPC title
Carriers for immersion in cryogenic fluid for slow freezing or vitrification · CPC title
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