Mogrol glycosyltransferase and gene encoding same

Patent metadata

Field	Value
Publication number	US-10407706-B2
Application number	US-201515519326-A
Country	US
Kind code	B2
Filing date	Oct 16, 2015
Priority date	Oct 17, 2014
Publication date	Sep 10, 2019
Grant date	Sep 10, 2019

Abstract

To provide a mogrol glycosyltransferase and a method for producing a mogrol glycoside using the enzyme. The present invention provides a mogrol glycosyltransferase and a method for producing a mogrol glycoside using the enzyme, and a transformant into which a mogrol glycosyltransferase gene is introduced and a method for preparing the transformant.

First claim

The invention claimed is:

1. A method for producing a mogrol glycoside comprising reacting a protein selected from the group consisting of the following (a) to (c), a UDP-sugar, and the compound represented by formula (I) to obtain a mogrol glycoside; and purifying the mogrol glycoside:
(a) a protein consisting of the amino acid sequence of SEQ ID NO: 2;
(b) a protein consisting of an amino acid sequence in which 1 to 45 amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2 and having an activity of adding a sugar molecule to an R 1 group at position 24 of a compound represented by the following formula (I); and
(c) a protein having an amino acid sequence having 90% or more sequence identity with the amino acid sequence of SEQ ID NO: 2 and having the activity of adding a sugar molecule to an R 1 group at position 24 of the compound represented by formula (I),

where R and R 1 each independently represent H, a monosaccharide, or a disaccharide.

2. The method according to claim 1 , wherein the UDP-sugar is a UDP-hexose.

3. The method according to claim 1 , wherein the UDP-sugar is selected from the group consisting of UDP-glucose, UDP-mannose, and UDP-galactose.

4. The method according to claim 1 , wherein the R and R 1 is each independently H or a sugar residue of a glucose monomer or a glucose dimer.

5. The method according to claim 1 , wherein the compound represented by formula (I) is mogrol or a mogrol glycoside.

6. The method according to claim 1 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside II 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.

7. The method according to claim 2 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.

8. The method according to claim 3 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.

9. The method according to claim 4 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1, mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.

10. The method according to claim 5 , which yields mogroside IA, mogroside Ib, mogroside IE, mogroside IIA, mogroside IIA 1 , mogroside IIE, mogroside III, mogroside IIIA 1 , mogroside IIIA 2 , mogroside IIIE, mogroside IV, mogroside IVA, mogroside V, siamenoside I, 11-oxomogroside V, mogrol pentaglycoside, or a combination thereof.

Assignees

• Suntory Holdings Ltd

Inventors

• Ono Eiichiro
• Ochiai Misa

Classifications

• C12N15/63

  Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12N1/16

  Yeasts; Culture media therefor · CPC title

• C12P33/00

  Preparation of steroids · CPC title