Peptide having titanium-binding ability

Patent metadata

Field	Value
Publication number	US-10406264-B2
Application number	US-201715545679-A
Country	US
Kind code	B2
Filing date	Mar 7, 2017
Priority date	Jul 26, 2016
Publication date	Sep 10, 2019
Grant date	Sep 10, 2019

Abstract

The present invention relates to a peptide having the ability to bind specifically to the surface of titanium. The titanium-binding peptide according to the present invention is fixed securely to the titanium surface so that the activity of a physiologically active substance linked to the peptide can be stably maintained over a long period of time. Thus, the peptide can be effectively used for surgical regeneration therapy.

First claim

The invention claimed is:

1. A titanium-binding peptide represented by the amino acid sequence of SLNYTGHRPVVH (SEQ ID NO: 1).

2. A peptide conjugate comprising a physiologically active peptide or protein linked to the titanium-binding peptide of claim 1 .

3. The peptide conjugate of claim 2 , wherein the physiologically active peptide is selected from the group consisting of anti-inflammatory, anti-microbial, cell adhesion, bone tissue regeneration, and cell migration functional peptides.

4. The peptide conjugate of claim 2 , wherein the physiologically active protein is selected from the group consisting of a tissue regeneration factor, a tissue growth factor, an intracellular transcription factor, an extracellular matrix protein, and an anti-inflammatory protein.

5. The peptide conjugate of claim 2 , wherein the peptide conjugate is represented by an amino acid sequence of SEQ ID NO: 3 or 4.

6. A biomaterial comprising the titanium-binding peptide of claim 1 or a peptide conjugate thereof, comprising a physiologically active peptide or protein linked to said titanium-binding peptide, wherein the biomaterial is any one selected from the group consisting of a titanium-coated metal, a titanium-containing metal, a titanium-coated natural polymer, a natural polymer mixed with titanium, a titanium-coated synthetic polymer, a synthetic polymer mixed with titanium, and an implant for in vivo implantation made of titanium.

7. The biomaterial of claim 6 , wherein the titanium is an alloy obtained by adding 5-7 wt % of aluminum, 3-5 wt % of vanadium, 0-0.25 wt % of iron and 0-0.2 wt % of oxygen to pure titanium oxide.

8. The biomaterial of claim 6 , wherein the natural polymer is any one selected from the group consisting of collagen, alginic acid, alginic acid, chondroitin sulfate, and chitosan.

9. The biomaterial of claim 6 , wherein the synthetic polymer is any one selected from the group consisting of polylacticglycolic acid, poloxamer, and propylene glycol.

Assignees

• Nano Intelligent Biomedical Eng Corporation Co Ltd
• Seoul Nat Univ R&Db Foundation

Inventors

• Chung Chong-Pyoung
• Park Yoon Jeong
• Lee Jue-Yeon
• Kim Yeonsu
• Lee Dong Woo

Classifications

• C07K17/14

  Peptides being immobilised on, or in, an inorganic carrier · CPC title

• A61L27/227

  Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24 · CPC title

• A61L27/54 Primary

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L27/22

  Polypeptides or derivatives thereof {, e.g. degradation products} · CPC title

• A61L27/06

  Titanium or titanium alloys · CPC title