Plant promoter for transgene expression

Patent metadata

Field	Value
Publication number	US-10400246-B2
Application number	US-201715698702-A
Country	US
Kind code	B2
Filing date	Sep 8, 2017
Priority date	Oct 3, 2016
Publication date	Sep 3, 2019
Grant date	Sep 3, 2019

Abstract

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a promoter from a Zea mays egg cell gene. Some embodiments relate to a promoter from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a 3′ UTR from a Zea mays egg cell gene that functions in plants to promote transcription of operably linked nucleotide sequences.

First claim

What is claimed is:

1. A nucleic acid vector comprising a promoter operably linked to a heterologous coding sequence of:
a) a polylinker sequence;
b) a non- Zea mays egg cell transgene; or
c) a combination of a) and b);
wherein said promoter comprises a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1.

2. The nucleic acid vector of claim 1 , wherein said promoter is 1,500 bp in length.

3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 95% sequence identity with SEQ ID NO:1.

4. The nucleic acid vector of any one of claim 1 , further comprising a sequence encoding a selectable marker.

5. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a heterologous coding sequence.

6. The nucleic acid vector of claim 5 , wherein the heterologous coding sequence encodes a selectable marker, an insecticidal resistance protein, a small RNA molecule, a site specific nuclease protein, a herbicide tolerance protein, a nitrogen use efficiency protein, a water use efficiency protein, a nutritional quality protein or a DNA binding protein.

7. The nucleic acid vector of claim 1 , further comprising a 3′ untranslated polynucleotide sequence.

8. The nucleic acid vector of claim 1 , further comprising a 5′ untranslated polynucleotide sequence.

9. The nucleic acid vector of claim 1 , further comprising an intron sequence.

10. The nucleic acid vector of claim 1 , wherein said promoter drives embryonic cell preferred expression of the heterologous coding sequence.

11. A transgenic plant comprising a polynucleotide sequence that has at least 98% sequence identity with SEQ ID NO:1 operably linked to a heterologous coding sequence, wherein said polynucleotide sequence promotes expression of the heterologous coding sequence.

12. The transgenic plant of claim 11 , wherein said plant is selected from the group consisting of Zea mays , wheat, rice, sorghum , oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.

13. The transgenic plant of claim 12 , wherein said plant is Zea mays.

14. The transgenic plant of claim 11 , further comprising a 3′ untranslated sequence.

15. The transgenic plant of claim 11 , wherein said heterologous coding sequence is preferentially expressed within embryonic cell tissue.

16. The transgenic plant of claim 11 , wherein said promoter is 1,500 bp in length.

Assignees

• Dow Agrosciences Llc

Inventors

• Kumar Sandeep
• Barone Pierluigi
• Hemingway Daren
• Etchison Emily
• Asberry Andrew
• Pence Heather
• Bowling Andrew J

Classifications

• C12N15/8234 Primary

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N15/8233 Primary

  Female-specific, e.g. pistil, ovule · CPC title

• C12N15/8209

  Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title