Compositions and methods for liquid mixing assessment

Patent metadata

Field	Value
Publication number	US-10392500-B2
Application number	US-201816050666-A
Country	US
Kind code	B2
Filing date	Jul 31, 2018
Priority date	Jun 27, 2012
Publication date	Aug 27, 2019
Grant date	Aug 27, 2019

Abstract

Compositions include an aqueous solution of an organic dye of molecular weight in the range of about 300 to about 1,000 and a density-enhancing material. An amount of the density-enhancing material in the aqueous solution is sufficient to achieve a density of about 1.0 to about 1.3 g/mL. The composition has a viscosity of about 3.5 to about 5.0 centipoise. The compositions are useful in assessing the adequacy of a liquid handling mixing device to mix a reaction mixture.

First claim

What is claimed is:

1. A composition consisting of an aqueous solution of an organic dye of molecular weight in the range of about 300 to about 1,000 Dalton and a density-enhancing material wherein an amount of the density-enhancing material in the aqueous solution is sufficient to achieve a density of about 1.0 to about 1.3 g/mL and wherein the composition has a viscosity of about 3.5 to about 5.0 centipoise, wherein the organic dye is
an aromatic azo dye, or
a triarlymethane dye, or is
selected from the group consisting of Erioglaucine, Ponceau S, Allura Red, Acid Red, Malachite Green, and Amaranth.

2. The composition according to claim 1 wherein the density-enhancing material is a polyhydroxy compound, or
a polyol, a monosaccharide or a disaccharide, or is
selected from the group consisting of sucrose, fructose, trehalose and a mixture of two or more thereof.

3. The composition according to claim 1 further consisting of one or both of a buffer and a preservative.

4. A method of assessing the adequacy of a test mixing device to mix a reaction mixture, the method comprising:
subjecting a layer of water adjacent a layer of the composition of claim 1 in a container to mixing using the test mixing device at a predetermined level of intensity and duration and measuring a first absorbance from the mixture at a predetermined wavelength;
subjecting a layer of water adjacent a layer of the composition of claim 1 in a container to mixing using a control mixing device at a level of intensity and duration that maximizes mixing of the mixture and measuring a second absorbance from the mixture at the predetermined wavelength; and
determining a ratio of the first absorbance to the second absorbance wherein a ratio outside the range of 0.95 to 1.05 indicates that the ability of the test mixing device to mix a reaction mixture at the predetermined intensity level and duration is not adequate.

5. The composition according to claim 1 , wherein the organic dye is an aromatic azo dye and wherein the density-enhancing material is a monosaccharide or disaccharide wherein the amount of monosaccharide or disaccharide in the aqueous solution is sufficient to achieve a density of about 1.1 to about 1.2 g/mL and wherein the composition has a viscosity of about 3.5 to about 5.0 centipoise.

6. The composition according to claim 5 wherein the organic dye is a triarylmethane azo dye or a fused aryl ring azo dye.

7. The composition according to claim 5 wherein the monosaccharide or disaccharide is selected from the group consisting of sucrose, fructose, trehalose and a mixture of two or more thereof.

8. The composition according to claim 5 further consisting of one or both of a buffer and a preservative.

9. A method of assessing the adequacy of a test mixing device to mix a reaction mixture, the method comprising:
subjecting a layer of water above a layer of the composition of claim 6 in a container to mixing using the test mixing device at a predetermined intensity level and duration and measuring a first absorbance from the mixture at a predetermined wavelength,
subjecting a layer of the composition of claim 6 adjacent a layer of water in a container to mixing using a control mixing device at an intensity level and duration that maximizes mixing of the mixture and measuring a second absorbance from the mixture at the predetermined wavelength; and
determining a ratio of the first absorbance to the second absorbance wherein a ratio outside the range of 0.95 to 1.05 indicates that the ability of the test mixing device to mix a reaction mixture at the predetermined level of intensity and duration is not adequate.

10. The method according to claim 9 wherein a ratio less than 0.95 indicates that the ability of the test mixing device to mix a reaction mixture at the predetermined intensity level and duration is not adequate.

11. The method according to claim 9 wherein the test mixing device is part of an automated analyzer.

12. The method according to claim 9 wherein the organic dye is selected from the group consisting of Ponceau S, Allura Red, Acid Red, and Amaranth and the density-enhancing material is selected from the group consisting of sucrose, fructose, trehalose and mixtures of two or more thereof.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Snyder Jason
• Carter Jr Richard H
• Nichols Patricia
• Dunfee William D

Classifications

• C09B67/0083 Primary

  Solutions of dyes · CPC title

• C08K5/41

  Compounds containing sulfur bound to oxygen · CPC title

• C08K5/053

  Polyhydroxylic alcohols · CPC title

• G01N35/00663

  of consumables · CPC title

• C08L5/00 Primary

  Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00 · CPC title