High-capacity storage of digital information in DNA

Patent metadata

Field	Value
Publication number	US-10387301-B2
Application number	US-201414556213-A
Country	US
Kind code	B2
Filing date	Nov 30, 2014
Priority date	Jun 1, 2012
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).

First claim

What is claimed is:

1. A method of storing an item of information in a plurality of DNA segments, the method comprising:
encoding bytes in an item of information stored in a first computer file as a DNA sequence data using a representation schema to represent the encoded bytes as at least one DNA nucleotide datum in the DNA sequence data;
splitting the DNA sequence data into a plurality of overlapping DNA segments data, wherein the plurality of overlapping DNA segments data build in a redundancy of storage of the items of information;
adding indexing information to the plurality of DNA segments data, the indexing information indicating a position in the DNA sequence data of any one nucleotide datum in any one of the plurality of DNA segments data;
synthesizing, by providing the plurality of DNA segments data as input to a computer-controlled DNA synthesis platform, a plurality of DNA segments.

2. The method of claim 1 , further including the addition of adapters data to the DNA segments data.

3. The method of claim 1 using a base-3 scheme for encoding the bytes.

4. The method of claim 1 , wherein the representation schema used is designed such that adjacent ones of the DNA nucleotide data are different.

5. The method of claim 1 , further comprising adding a parity-check to the indexing information.

6. The method of claim 1 , wherein alternate ones of the DNA segments data are reverse complemented.

7. The method of claim 1 , wherein the representation schema used is designed to avoid long, self-reverse complementary DNA segments data.

8. The method of claim 1 , further comprising:
storing the synthesized DNA segments in one or more DNA archives.

9. A plurality of DNA segments that store an item of information, wherein the plurality of DNA segments data are produced by a method comprising:
encoding bytes in an item of information, stored in a first computer file, as a DNA sequence data using a representation schema to represent the encoded bytes as at least one DNA nucleotide datum in the DNA sequence data;
splitting the DNA sequence data into a plurality of overlapping DNA segments data, wherein the plurality of overlapping DNA segments data build in a redundancy of storage of the items of information;
augmenting indexing information to the plurality of DNA segments data, the indexing information indicating a position in the DNA sequence data of any one nucleotide datum in any one of the plurality of DNA segments data; and
synthesizing, by providing the plurality of DNA segments data as input to a computer-controlled DNA synthesis platform, a plurality of DNA segments.

Assignees

• Goldman Nick
• Birney John
• European Molecular Biology Laboratory

Inventors

• Goldman Nick
• Birney John

Classifications

• G06F2212/1032

  Reliability improvement, data loss prevention, degraded operation etc · CPC title

• G16B50/50

  Compression of genetic data · CPC title

• B82Y10/00 Primary

  Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic · CPC title

• G06N3/123 Primary

  DNA computing · CPC title

• G06F12/023 Primary

  Free address space management · CPC title