Particle manipulation and trapping in microfluidic devices using two-dimensional material

Patent metadata

Field	Value
Publication number	US-10386331-B2
Application number	US-201615093816-A
Country	US
Kind code	B2
Filing date	Apr 8, 2016
Priority date	Apr 8, 2016
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

Method, apparatus, and computer program product for a microfluidic channel having a cover opposite its bottom and having electrodes with patterned two-dimensional conducting materials, such as graphene sheets integrated into the top of its bottom. Using the two-dimensional conducting materials, once a fluid sample is applied into the channel, highly localized modulated electric field distributions are generated inside the channel and the fluid sample. This generated field causes the inducing of dielectrophoretic (DEP) forces. These DEP forces are the same or greater than DEP forces that would result using metallic electrodes because of the sharp edges enabled by the two-dimension geometry of the two-dimensional conducting materials. Because of the induced forces, micro/nano-particles in the fluid sample are separated into particles that respond to a negative DEP force and particles that respond to a positive DEP. Microfluidic chips with microfluidic channels can be made using standard semiconductor manufacturing technology.

First claim

What is claimed is:

1. A method, comprising:
applying a fluid sample into a microfluidic channel, wherein the micro fluidic channel comprises a cover opposite the bottom, wherein integrated into the top of bottom of the microfluidic channel are electrodes, wherein the electrodes comprise patterned two-dimensional conducting materials, wherein electrical contact of the patterned two-dimensional conducting materials is ensured;
generating highly localized modulated electric field distribution inside the channel and the fluid sample using the two-dimensional conducting materials;
based on the generating, inducing DEP forces, wherein the DEP forces are the same or greater than DEP forces that would use metallic electrodes due to the sharp edges enabled by the two-dimension geometry of the two-dimensional conducting materials;
in response to the inducing, separating micro/nano-particles in the fluid sample into particles that respond to a negative DEP force and particles that respond to a positive DEP; and
in response to the particles interacting with the electrodes, separating particles via side channels by size.

2. The method of claim 1 , wherein the two-dimensional conducting materials comprise graphene sheets.

3. The method of claim 1 , wherein a microfluidic chip comprises the microfluidic channel.

4. The method of claim 3 , wherein microfluidic chips can be fabricated and patterned using standard semiconductor manufacturing technology.

5. The method of claim 3 , wherein the microfluidic chip further comprises a generator for the generating.

6. The method of claim 1 , wherein the two-dimensional conducting materials can be patterned into any arbitrary shape inside and outside the channel, wherein the two-dimensional conducting materials acting as two-dimensional electrodes create a same or improved electric field modulation locally than metallic electrodes.

7. The method of claim 1 , further comprising:
designing the channel and voltage for the negative DEP force experienced by the particle to be stronger than the hydrodynamic drag;
trapping particles in place with conventional negative DEP levitation on top of the electrode and under the cover; and
detecting the trapped particles.

8. The method of claim 1 , further comprising:
determining, in response to the separating, numbers of particles that respond to a negative DEP force and of particles that respond to a positive DEP, wherein the determining can be done by at least one of:
a microfluidic chip comprising the microfluidic channel and a microprocessor, and
a device connecting to a microfluidic chip comprising the microfluidic channel, wherein the connecting can be a physical connection or a wireless connection.

9. The method of claim 8 , further comprising:
outputting results of the determining.

10. An apparatus, comprising:
a micro fluidic channel where a fluid sample can be applied into, wherein the micro fluidic channel comprises a cover opposite the bottom, wherein integrated into the top of bottom of the microfluidic channel are electrodes, wherein the electrodes comprise patterned two-dimensional conducting materials, wherein electrical contact of the patterned two-dimensional conducting materials is ensured; and
a generator to generate highly localized modulated electric field distribution inside the channel and the fluid sample using the two-dimensional conducting materials,
wherein based on said generating, DEP forces are induced, wherein the DEP forces are the same or greater than DEP forces that would use metallic electrodes due to the sharp edges enabled by the two-dimension geometry of the two-dimensional conducting materials,
wherein in response to said inducing, micro/nano-particles in the fluid sample are separated into particles that respond to a negative DEP force and particles that respond to a positive DEP, and
and wherein in response to the particles interacting with the electrodes, separating particles via side channels by size.

11. The apparatus of claim 10 , wherein the two-dimensional conducting materials comprise graphene sheets.

12. The apparatus of claim 11 , wherein microfluidic chips can be fabricated and patterned using standard semiconductor manufacturing technology.

13. The apparatus of claim 10 , wherein a microfluidic chip comprises the apparatus.

14. The apparatus of claim 10 , further comprising: at least one processor; and at least one memory including computer program code, wherein the at least one processor, in response to execution of the computer program code, is configured to cause the apparatus to control or perform at least one of:
depositing the fluid sample into the microfluidic channel;
generating the highly localized field distribution; and
in response said separating of particles, calculating a number of particles separated.

15. The apparatus of claim 10 , wherein the two-dimensional conducting materials can be patterned into any arbitrary shape inside and outside the channel, wherein the two-dimensional conducting materials acting as two-dimensional electrodes create a same or improved electric field modulation locally than metallic electrodes.

16. The apparatus of claim 10 ,
wherein the channel and voltage for the negative DEP force experienced by the particle are designed to be stronger than the hydrodynamic drag;
wherein conventional negative DEP levitation on top of the electrode and under the covert to trap particles in place with; and
further comprising a detector for detecting the trapped particles.

17. The apparatus of claim 10 , further comprising at least one of:
an antenna, a transmitter, and associated circuitry to transmit wirelessly to a device;
an antenna, a received to communicate wirelessly to a device; and
circuitry to connect physically with a device.

18. A computer program product comprising a computer-readable medium bearing computer program code embodied therein for use with a computer, the computer program code comprising code for performing or controlling:
applying a fluid sample into a microfluidic channel, wherein the microfluidic channel comprises a cover opposite the bottom, wherein integrated into the top of bottom of the microfluidic channel are electrodes, wherein the electrodes comprise patterned two-dimensional conducting materials, wherein electrical contact of the patterned two-dimensional conducting materials is ensured;
generating highly localized modulated electric field distribution inside the channel and the fluid sample using the two-dimensional conducting materials;
based on the generating, inducing DEP forces, wherein the DEP forces are the same or greater than DEP forces that would use metallic electrodes due to the sharp edges enabled by the two-dimension geometry of the two-dimensional conducting materials;
in response to the inducing, separating micro/nano-particles in the fluid sample into particles that respond to a negative DEP force and particles that respond to a positive DEP; and
in response to the particles interacting with the electrodes, separating particles via side channels by size.

19. An apparatus comprising:
means for accepting an application of a fluid sample into a microfluidic channel, wherein the microfluidic channel comprises a cover opposite the bottom, wherein integrated into the top of bottom of the microfluidic channel are electrodes, wherein the electrodes comprise patterned two-dimensional conducting materials, wherein electrical contact of the patterned two-dimensional conducting materials is ensured;
means for genera

Assignees

• IBM

Inventors

• Azpiroz Jaione Tirapu
• Engel Michael
• Steiner Mathias B

Classifications

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• G01N27/44704

  Details; Accessories · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title