Systems and methods for a multi-chambered sampler

Patent metadata

Field	Value
Publication number	US-10386278-B2
Application number	US-201715596946-A
Country	US
Kind code	B2
Filing date	May 16, 2017
Priority date	May 17, 2016
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

A system for mixing a sample with a combined buffer includes a sampler body, the sampler body including a first reservoir and a second reservoir. The system further includes a first separator forming a first enclosure with the sampler body for the first reservoir. The system further includes a second separator forming a second enclosure with the sampler body for the first reservoir. The system further includes a third separator, in conjunction with the second separator, forming a third enclosure and a fourth enclosure, respectively, both in conjunction with the sampler body, for the second reservoir.

First claim

What is claimed as new and desired to be protected by Letters Patent of the United States is:

1. A system for mixing a sample with a combined buffer, the system comprising:
a sampler body, the sampler body including a first reservoir and a second reservoir;
a first separator forming a first enclosure with the sampler body for the first reservoir;
a second separator forming a second enclosure with the sampler body for the first reservoir;
a third separator, in conjunction with the second separator, forming a third enclosure and a fourth enclosure, respectively, both in conjunction with the sampler body, for the second reservoir,
a first piercing element configured to pierce the first and second separators; and
a second piercing element configured to pierce the third separator.

2. The system of claim 1 , wherein the first and third separators are foil.

3. The system of claim 2 , wherein the second separator is a septum.

4. The system of claim 3 , wherein the first reservoir includes a first buffer and the second reservoir includes a second buffer.

5. The system of claim 4 , further comprising:
a blood collector, the blood collector including the first piercing projection, the blood collector shaped to mate with the sampler body, such that when the blood collector is inserted into the sampler body at an aperture in the sampler body, liquid cannot escape from the combination of the sampler body and the blood collector.

6. The system of claim 5 , wherein upon insertion of the blood collector into the sampler body, the first piercing projection of the sampler body pierces the first and second separators.

7. The system of claim 6 , wherein the sampler body includes a capillary tube that holds the sample.

8. The system of claim 7 , wherein upon insertion of the blood collector into the sampler body, the first and second buffers mix with the sample.

9. The system of claim 8 , wherein the sampler body includes a cylindrical cavity that houses the first and second reservoirs.

10. The system of claim 9 , wherein the septum provides one pound of force resistance to piercing.

11. The system of claim 9 , wherein the septum has four equal quadrants joined by a thin connector material.

12. The system of claim 11 , wherein the septum is molded plastic.

13. The system of claim 12 , wherein the four equal quadrants are thicker that the thin connector material.

14. A method for mixing a sample with a combined buffer, the method comprising:
providing a sampler body, the sampler body including:
a first reservoir and a second reservoir;
a first separator forming a first enclosure with the sampler body for the first reservoir;
a second separator forming a second enclosure with the sampler body for the first reservoir; and
a third separator, in conjunction with the second separator, forming a third enclosure and a fourth enclosure, respectively, both in conjunction with the sampler body, for the second reservoir;

providing a blood collector, the blood collector including a first piercing projection, the blood collector shaped to mate with the sampler body, such that when the blood collector is inserted into the sampler body at an aperture in the sampler body, liquid cannot escape from the combination of the sampler body and the blood collector;
inserting the blood collector into the sampler body;
advancing the blood collector and breaking the first separator with the first piercing projection;
advancing the blood collector and breaking the second separator with the first piercing projection;
connecting a sampler base to the sampler body, the sampler base including a plunger and a second piercing projection; and
advancing the sampler base and breaking the third separator with the second piercing projection.

15. The method of claim 14 , wherein the first reservoir includes a first buffer and the second reservoir includes a second buffer.

16. The method of claim 14 , further comprising:
mixing the first and second buffers; and
mixing a sample held in the blood collector with the first and second buffers by agitating the combined sampler body and blood collector.

17. The method of claim 16 , wherein the first and third separators are foil.

18. The method of claim 17 , wherein the second separator is a septum.

19. The method of claim 18 , wherein the sampler body includes a capillary tube that holds the sample.

20. The method of claim 19 , wherein the septum has four equal quadrants joined by a thin connector material.

21. The method of claim 20 , wherein the septum is molded plastic.

22. The method of claim 21 , wherein the four equal quadrants are thicker than the thin connector material.

23. The system of claim 1 , further comprising a sampler base connected to the sampler body, the sampler base including a plunger and the second piercing element.

24. The system of claim 5 , wherein the sampler body includes opposing open ends, one end open to receive the blood collector, the other end open to release the combined buffer onto a test strip when the third separator is pierced by the second piercing element.

25. The system of claim 11 , wherein the thin connector material is a weakened region of material connected to the four equal quadrants, the thin connector material breaking upon insertion of the blood collector.

26. A system for mixing a sample with a combined buffer, the system comprising:
a sampler body, the sampler body including a first reservoir and a second reservoir;
a first separator forming a first enclosure with the sampler body for the first reservoir;
a second separator forming a second enclosure with the sampler body for the first reservoir, the second separator also forming a first enclosure with the sampler body for the second reservoir;
a third separator forming a second enclosure with the sampler body for the second reservoir; and
a sampler base connected to the sampler body, the sampler base including a plunger and a piercing element.

Assignees

• Polymer Technology Systems Inc

Inventors

• Pierce Jeffrey A
• Phan Bao
• Ramel Urs A

Classifications

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title

• G01N1/38 Primary

  Diluting, dispersing or mixing samples · CPC title

• B01L3/502

  with fluid transport, e.g. in multi-compartment structures · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title

• B01L2300/0832

  cylindrical, tube shaped · CPC title