Processes for producing fermentation products

Patent metadata

Field	Value
Publication number	US-10385374-B2
Application number	US-201815913092-A
Country	US
Kind code	B2
Filing date	Mar 6, 2018
Priority date	Sep 11, 2013
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and a thermostable endoglucanase is present and/or added during liquefaction. The invention also relates to compositions suitable for use in processes of the invention.

First claim

The invention claimed is:

1. A process for producing ethanol from starch-containing material comprising the steps of:
i) liquefying the starch-containing material at a temperature from 75-95° at a pH from 4-6 using:
an alpha-amylase; and
a polypeptide having endoglucanase activity and a Melting Point (DSC) above 80° C., wherein the polypeptide is a GH5 family endoglucanase;

ii) saccharifying using a glucoamylase; and
iii) fermenting using a fermenting organism.

2. The process of claim 1 , wherein the alpha-amylase has at least 90% sequence identity to the polypeptide of SEQ ID NO: 1.

3. The process of claim 2 , wherein the alpha-amylase has at least 95% sequence identity to the polypeptide of SEQ ID NO: 1.

4. The process of claim 1 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl 2 ) of at least 10.

5. The process of claim 1 , further wherein a protease is present or added in liquefaction, wherein the protease has a thermostability value of more than 25% determined as Relative Activity at 80° C./70° C.

6. The process of claim 1 , wherein the protease has at least 90%, but less than 100%, sequence identity to the mature polypeptide of SEQ ID NO: 2.

7. The process of claim 6 , wherein the protease has at least 90% sequence identity to the polypeptide of SEQ ID NO: 13.

8. The process of claim 6 , wherein the protease has at least 95% sequence identity to the polypeptide of SEQ ID NO: 13.

9. The process of claim 1 , further glucoamylase having a heat stability at 85° C., pH 5.3, of at least 20% is present or added during liquefaction step i).

10. The process of claim 9 , wherein the starch-containing material is corn.

11. The process of claim 9 , wherein the glucoamylase has at least 90% sequence identity to the polypeptide of SEQ ID NO: 9 or at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 14.

12. The process of claim 9 , wherein the glucoamylase has at least 95% sequence identity to the polypeptide of SEQ ID NO: 9 or SEQ ID NO: 14.

13. A process of producing liquefied starch, comprising liquefying a starch-containing material with a composition comprising:
i) an alpha-amylase;
ii) a polypeptide having endoglucanase activity and a Melting Point (DSC) above 80° C., wherein the polypeptide is a GH5 family endoglucanase;
iii) a protease; and
iv) optionally a glucoamylase.

14. The process of claim 13 , wherein the alpha-amylase has at least 90% sequence identity to the polypeptide of SEQ ID NO: 1.

15. The process of claim 13 , wherein the alpha-amylase has a T½ (min) at pH 4.5, 85° C., 0.12 mM CaCl 2 ) of at least 10.

16. The process of claim 13 , wherein the protease has a thermostability value of more than 25% determined as Relative Activity at 80° C./70° C.

17. The process of claim 13 , wherein the protease has at least 90%, but less than 100%, sequence identity to the mature polypeptide of SEQ ID NO: 2.

18. The process of claim 13 , wherein the protease has at least 90% sequence identity to the polypeptide of SEQ ID NO: 13.

19. The process of claim 13 , wherein the carbohydrate-source generating enzyme is present and is a glucoamylase having a heat stability at 85° C., pH 5.3, of at least 20%.

20. The process of claim 19 , wherein the glucoamylase has at least 90% sequence identity to the polypeptide of SEQ ID NO: 9 or at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 14.

Assignees

• Novozymes As

Inventors

• Kang Zhengfang
• Strahler Christie
• Clark Suzanne
• Krogh Kristian Bertel Rømer M
• Lan Tang

Classifications

• C12Y302/01006

  Endo-1,3(4)-beta-glucanase (3.2.1.6) · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C12Y302/01003

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title

• C12N9/244

  Endo-1,3(4)-beta-glucanase (3.2.1.6) · CPC title