Fungi and products thereof
US-9222096-B2 · Dec 29, 2015 · US
Method for producing polyisoprenoid, transformed plant, method for producing pneumatic tire and method for producing rubber product
US10385362B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10385362-B2 |
| Application number | US-201615170584-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 1, 2016 |
| Priority date | Jun 30, 2015 |
| Publication date | Aug 20, 2019 |
| Grant date | Aug 20, 2019 |
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- Title
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Abstract
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Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing a rubber, the method comprising: a step of binding a protein expressed by a gene coding for a cis-prenyltransferase (CPT) family protein, a protein expressed by a gene coding for a Nogo-B receptor (NgBR) family protein, and a protein expressed by a gene coding for a rubber elongation factor (REF) family protein to rubber particles in vitro, wherein the gene coding a for a cis-prenyltransferase (CPT) family protein, the gene coding for a Nogo-B receptor (NgBR) family protein, and the gene coding for a rubber elongation factor (REF) family protein are each derived from plants, and wherein the cis-prenyltransferase (CPT) family protein is at least one selected from the group consisting of CPT from Hevea brasiliensis (HRT1, HRT2, CPT3-5), AtCPTl-9 from Arabidopsis thaliana , CPT 1-3 from lettuce, and CPT 1-3 from Taraxacum kok - saghyz , and wherein the Nogo-B receptor (NgBR) family protein is at least one selected from the group consisting of NgBR from Hevea brasiliensis (HRTBP), LEW1 from Arabidopsis thaliana , LsCPTLl-2 from lettuce, and TbRTA from Taraxacum , and wherein the rubber elongation factor (REF) family protein is REF from Hevea brasiliensis. 2. The method for producing a rubber according to claim 1 , wherein the binding step comprises performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a cis-prenyltransferase (CPT) family protein, an mRNA coding for a Nogo-B receptor (NgBR) family protein, and an mRNA coding for a rubber elongation factor (REF) family protein to bind the CPT family protein, the NgBR family protein, and the REF family protein to the rubber particles. 3. The method for producing a rubber according to claim 2 , wherein the cell-free protein synthesis solution contains a germ extract. 4. The method for producing a rubber according to claim 3 , wherein the germ extract is derived from wheat. 5. The method for producing a rubber according to claim 2 , wherein the rubber particles are present in the cell-free protein synthesis solution at a concentration of 5 to 50 g/L. 6. The method for producing a rubber according to claim 2 , wherein each gene of the gene coding for a cis-prenyltransferase (CPT) family protein, the gene coding for a Nogo-B receptor (NgBR) family protein, and the gene coding for a rubber elongation factor (REF) family protein, is derived from Hevea brasiliensis.
Assignees
Inventors
Classifications
- C12P5/007Primary
containing one or more isoprene units, i.e. terpenes (carotenes C12P23/00) · CPC title
Vulcanising tyres; Vulcanising presses for tyres · CPC title
Rubber cis-polyprenylcistransferase (2.5.1.20) · CPC title
Use of natural rubber for preformed parts, e.g. for inserts · CPC title
from plants · CPC title
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- What does patent US10385362B2 cover?
- Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elonga…
- Who is the assignee on this patent?
- Sumitomo Rubber Ind, Univ Tohoku
- What technology area does this patent fall under?
- Primary CPC classification C12P5/007. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 20 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).