Extraction of lipid from cells and products therefrom

Patent metadata

Field	Value
Publication number	US-10385289-B2
Application number	US-201815875733-A
Country	US
Kind code	B2
Filing date	Jan 19, 2018
Priority date	Jun 1, 2010
Publication date	Aug 20, 2019
Grant date	Aug 20, 2019

Abstract

The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.

First claim

What is claimed is:

1. A process for obtaining a lipid from a cell composition, comprising:
(a) lysing a cell of said cell composition to form a lysed cell composition;
(b) demulsifying said lysed cell composition to form a demulsified cell composition, wherein the demulsifying comprises contacting said lysed cell composition with a first base to raise the pH of the lysed cell composition to 8 and further comprising contacting the lysed cell composition with a second base to raise the pH of the lysed cell composition to 8 or above to demulsify the cell composition;
(c) separating said lipid from the demulsified cell composition; and
(d) harvesting said lipid;
wherein (b) comprises heating said lysed cell composition to a temperature of at least 70° C.

2. The process of claim 1 , wherein (b) further comprises contacting said lysed cell composition with a salt.

3. The process of claim 2 , wherein the salt is added in an amount of 0.1% to 20% by weight of the lysed cell composition.

4. The process of claim 2 , wherein the salt is selected from the group consisting of alkali metal salts, alkali earth metal salts, sulfate salts, and combinations thereof.

5. The process of claim 1 , comprising washing the cell or cell composition prior to lysing the cell.

6. The process of claim 1 , comprising pasteurizing the cell or cell composition prior to lysing the cell.

7. The process of claim 1 , step (a) further comprising concentrating the lysed cell composition.

8. A lipid obtained by the process of claim 1 , the lipid comprising at least 35% by weight docosahexaenoic acid, at least 20% by weight eicosapentaenoic acid and less than 5% by weight each of arachidonic acid, docosapentaenoic acid n-6, oleic acid, linoleic acid, linolenic acid, eicosenoic acid, erucic acid, and stearidonic acid.

9. The lipid of claim 8 , wherein the lipid has an anisidine value of 26 or less.

10. The lipid of claim 8 , wherein the lipid has a phosphorus content of 100 ppm or less.

11. The lipid of claim 8 , wherein the lipid has a peroxide value of 5 or less.

12. The lipid of claim 8 , wherein the lipid has a peroxide value of 5 or less.

13. The lipid of claim 8 , wherein the lipid is a crude oil.

14. The process of claim 1 , wherein (b) comprises heating said lysed cell composition to a temperature of from 70° C. to 100° C.

15. The process of claim 1 , wherein (b) comprises agitating the lysed cell composition to demulsify the cell composition.

16. The process of claim 1 , wherein (a) comprises adding a salt to said cell composition.

17. The process of claim 1 , wherein (a) comprises raising the pH of said cell composition to 8 or above to lyse the cell composition.

18. The process of claim 1 , wherein the lysed cell composition is in the form of an oil-in-water emulsion comprising a mixture of a continuous aqueous phase and a dispersed lipid phase.

19. The process of claim 1 , wherein (c) comprises centrifuging the demulsified cell composition.

20. The process of claim 1 , wherein the lipid is a crude lipid.

21. The process of claim 20 , further comprising (e) refining said crude lipid to obtain a refined lipid.

22. The process of claim 1 , wherein said lipid has an anisidine value of 26 or less.

23. The process of claim 1 , wherein said lipid has a peroxide value of 5 or less.

24. The process of claim 1 , wherein said cell is a microbial cell.

25. The process of claim 24 , wherein said microbial cell is of the genus Thraustochytrium, Schizochytrium , or mixtures thereof.

26. The process of claim 1 , wherein said lipid comprises omega 3 and/or omega-6 polyunsaturated fatty acids.

27. The process of claim 1 , wherein said lipid comprises at least one of DHA, DPA(n-3), DPA(n-6), EPA, and ARA.

28. The process of claim 1 , wherein an organic solvent is not added to said cell composition, said lysed cell composition, or said lipid in an amount or concentration sufficient to extract said lipid from said cell.

29. The process of claim 1 , wherein the lipid contains less than 5% by weight of an organic solvent.

30. The process of claim 21 , wherein refining is selected from the group consisting of caustic refining, degumming, acid treatment, alkali treatment, cooling, heating, bleaching, deodorizing, deacidification, and combinations thereof.

Assignees

• Dsm Ip Assets Bv

Inventors

• Cherinko Stephen Robert
• Kertis Robert Cody
• Apt Kirk E
• Behrens Paul Warren
• Hansen Jon Milton
• Pfeifer Iii Joseph W
• Stahl Tracey Lynn
• Zirkle Ross
• Tabayehnejad Nasrin
• Raman Krishna
• Leininger Neil Francis

Classifications

• C11B1/10 Primary

  by extracting · CPC title

• C12P7/649

  Biodiesel, i.e. fatty acid alkyl esters · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P7/64 Primary

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title

• C11B3/16

  by mechanical means · CPC title