System and method for performing a chromatography injection sequence using a single injection valve

Patent metadata

Field	Value
Publication number	US-10379088-B2
Application number	US-201515527182-A
Country	US
Kind code	B2
Filing date	Nov 2, 2015
Priority date	Dec 1, 2014
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

A rotary valve used in chromatography includes a stator with a plurality of stator ports arranged on the stator. The stator further includes a stator groove connected at one end to a first stator port and terminating between the first stator port and a second stator port adjacent to the first stator port. A rotor, rotatably fitted to the stator, has a plurality of arcuate channels arranged in an asymmetrical pattern on the rotor. Each rotor channel connects to one or more of the stator ports. Different connections of the rotor channels to the stator ports produce at least three different positions for the injection valve. The three different positions provide a complete chromatography sample injection sequence using only a single valve.

First claim

What is claimed is:

1. A sample manager comprising:
a sample loop; and
a single injection valve configured to (i) depressurize the sample loop, (ii) draw a sample into the sample loop, and (iii) inject the sample in the sample loop into a pressurized fluidic stream, the injection valve having a stator and a rotor rotatably fitted to the stator, the stator having a plurality of stator ports arranged on the stator, the stator further including a stator groove connected at one end to a first one of the stator ports and terminating at an opposite end between the first stator port and a second one of the stator ports adjacent to the first stator port, the first stator port being fluidically coupled to a source of the pressurized fluidic stream, the second stator port being fluidically coupled to a destination of the pressurized fluidic stream, the rotor having a plurality of rotor grooves arranged in an asymmetrical pattern on the rotor, each of the rotor grooves connecting to one or more of the stator ports,
wherein one of the rotor grooves has a length sufficient to connect together four adjacent stator ports and wherein the injection valve has at least three different positions including a first position in which a first one of the rotor grooves connects the stator groove to the second stator port to enable the pressurized fluidic stream arriving at the first stator port to flow directly to the second stator port towards the destination while the sample loop depressurizes.

2. The sample manager of claim 1 , further comprising:
a flow-through needle connected to a third one of the stator ports;
a pressure source connected to a fourth one of the stator ports, and
wherein the at least three different positions of the injection valve includes a second position in which the first rotor groove connects the first stator port to the second stator port to enable the pressurized fluidic stream arriving at the first stator port to flow directly to the second stator port and a second one of the rotor grooves connects the third stator port to the fourth stator port to enable the pressure source to draw the sample from a sample source into the flow-through needle.

3. The sample manager of claim 2 , further comprising:
a seat into which the flow-through needle is driven and sealed, an outlet of the seat being connected to a fifth one of the stator ports, and
wherein the at least three different positions of the injection valve includes a third position in which the second rotor groove connects the first stator port to the third stator port to enable the pressurized fluidic stream arriving at the first stator port to flow into the flow-through needle containing the sample and the first rotor groove connects the fifth stator port to the second stator port to enable the pressurized fluidic stream containing the sample to exit the sample loop and flow towards the destination.

4. The sample manager of claim 3 , wherein, while the injection valve is in the second position and the flow-through needle is driven into and sealed by the seat, the pressure source pressurizes the sample loop containing the sample before the injection valve moves to the third position to inject the sample to the pressurized fluidic stream.

5. The sample manager of claim 1 , further comprising:
a flow-through needle connected to a third one of the stator ports adjacent the first stator port;
a waste receptacle connected to a fourth one of the stator ports;
a pressure source connected to a fifth one of the stator ports disposed between the third and fourth stator ports;
a seat into which the flow-through needle is driven and sealed, an outlet of the seat being connected to a sixth one of the stator ports; and
wherein the at least three different positions of the injection valve includes a second position in which the sixth stator port is blocked, thereby blocking a flow through the sample loop, and wherein a second one of the rotor grooves connects the first, third, fourth, and fifth stator ports to enable the pressurized fluidic stream arriving at the first stator port to flow towards the waste receptacle through the fourth stator port so the pressure source can be primed.

6. The sample manager of claim 1 , wherein the asymmetrical pattern of the rotor grooves on the rotor is produced by at least one of the rotor grooves having a different length than at least one of the other rotor grooves.

7. The sample manager of claim 1 , wherein the asymmetrical pattern of the rotor grooves on the rotor is produced by at least one gap between neighboring rotor grooves being greater than at least one other gap between neighboring rotor grooves.

8. The sample manager of claim 1 , wherein the stator has eight stator ports.

9. A method of completely performing a chromatography injection sequence using only a single rotary valve having a stator and a rotor rotatably fitted to the stator, the stator having a plurality of stator ports arranged on the stator, the stator further including a stator groove connected at one end to a first one of the stator ports, an opposite end of the stator groove terminating between the first stator port and a second one of the stator ports that is adjacent to the first stator port, a third one of the stator ports being connected to an inlet of a sample loop, a fourth one of the stator ports being connected to an outlet of the sample loop, the rotor having a plurality of rotor grooves arranged in an asymmetrical pattern on the rotor, each of the rotor grooves connecting to one or more of the stator ports, the method comprising:
delivering a pressurized fluidic stream to the first stator port;
fluidically coupling the second stator port to a destination of the pressurized fluidic stream;
placing the rotary valve into a first position in which a first one of the rotor grooves connects the stator groove to the second stator port to cause the pressurized fluidic stream arriving at the first stator port to flow through the stator groove directly to the second stator port and towards the destination, while the first position of the rotary valve blocks the inlet and outlet of the sample loop, enabling the sample loop to depressurize;
changing the rotary valve from the first position to a second position in which the first rotor groove connects the first stator port to the second stator port to cause the pressurized fluidic stream arriving at the first stator port to flow directly to the second stator port, while enabling a sample to be drawn into the sample loop; and
changing the rotary valve, by turning the rotary valve one-half step, from the second position to a third position to redirect the pressurized fluidic stream arriving at the first stator port through the sample loop containing the sample before the pressurized fluidic stream flows to the second stator port towards the destination.

10. The method of claim 9 , wherein changing the rotary valve from the second position to the third position includes turning the rotary valve one full step in a direction opposite a direction used to change the rotary valve from the first position to the second position.

11. The method of claim 9 , further comprising pressurizing the sample loop containing the sample while the rotary valve is in the second position before changing the rotary valve to the third position to inject the sample into the pressurized fluidic stream.

12. The method of claim 9 , further comprising:
connecting a flow-through needle to a third one of the stator ports;
connecting a pressure source to a fourth one of the stator ports; and
wherein, while the rotary valve is in the second position, a second one of the rotor grooves connects the th

Assignees

• Waters Technologies Corp

Inventors

• Cormier Sylvain

Classifications

• G01N2030/202

  rotary valves · CPC title

• G01N2030/207

  with metering cavity, e.g. sample loop · CPC title

• G01N30/20 Primary

  using a sampling valve · CPC title

• G01N2030/201

  multiport valves, i.e. having more than two ports · CPC title