Effervescent compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-10378041-B2
Application number	US-201615002696-A
Country	US
Kind code	B2
Filing date	Jan 21, 2016
Priority date	Dec 31, 2010
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.

First claim

The invention claimed is:

1. A method of enriching the growth of a target microorganism, comprising:
providing a culture medium, a sample that includes the target microorganism, and a latent effervescent body;
wherein the latent effervescent body comprises an outer shell with a core composition disposed therein;
wherein the core composition comprises two or more effervescent components and a selective agent that facilitates the growth of the target microorganism relative to growth of at least one microorganism other than the target microorganism;

contacting the sample, the latent effervescent body, and the culture medium under aqueous conditions to facilitate growth of the target microorganism;
allowing the target microorganism to grow over a time period of about 2 hours to about 6 hours; and
releasing the selective agent from the latent effervescent body after a time period of about 2 hours to about 6 hours;
wherein releasing the selective agent further comprises distributing the selective agent in the culture medium;
wherein distributing the selective agent comprises distributing the selective agent via a mixing process resulting from gaseous effervescence;
wherein releasing the selective agent comprises delaying the release of the selective agent for a predetermined time after the step of contacting the sample, the latent effervescent body, and the culture medium under aqueous conditions.

2. The method of claim 1 , wherein contacting the sample, the latent effervescent body, and the culture medium further comprises contacting the sample with an effective amount of released selective agent for a period of time sufficient to permit selective enrichment of the target microorganism.

3. The method of claim 1 , further comprising detecting a microorganism.

4. The method of claim 3 , wherein detecting the microorganism comprises propagating the microorganism on or in a semisolid culture medium, observing the microorganism microscopically, detecting metabolic activity of the microorganism, detecting a biomolecule of the microorganism, or detecting a nucleic acid of the microorganism.

Assignees

• 3M Innovative Properties Co

Inventors

• Xia Wensheng
• Mach Patrick A
• Beaurline Joseph M
• Bjork Jason W
• Liu Jie J

Classifications

• C12Q1/045 Primary

  Culture media therefor · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12N1/14 Primary

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• C12N1/38

  Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) · CPC title