Method of producing chemical substance

Patent metadata

Field	Value
Publication number	US-10378029-B2
Application number	US-201314371860-A
Country	US
Kind code	B2
Filing date	Jan 11, 2013
Priority date	Jan 13, 2012
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

A method produces a chemical product by continuous fermentation including filtering a culture liquid of a microorganism(s) through a separation membrane, retaining unfiltered liquid in, or refluxing unfiltered liquid to, the culture liquid, adding a fermentation feedstock to the culture liquid, and recovering a product in the filtrate, wherein the microorganism(s) is/are a microorganism(s) that undergo(es) catabolite repression, and the fermentation feedstock comprises hexose and pentose.

First claim

The invention claimed is:

1. A method of producing a chemical product by continuous fermentation comprising:
filtering a culture liquid of a microorganism(s) comprising yeast or bacteria through a separation membrane;
retaining unfiltered liquid in, or refluxing unfiltered liquid to, the culture liquid;

continuously adding a fermentation feedstock comprising a mixed sugar of hexose and pentose to the culture liquid; and
recovering a product in the filtrate;
wherein
said microorganism(s) is/are a microorganism(s) that metabolizes(s) hexose and pentose and undergo(es) catabolite repression, whose consumption of pentose is suppressed when a fermentation feedstock comprising a mixed sugar containing hexose and pentose is used,
the total sugar concentration in the fermentation feedstock is 60 g/L to 500 g/L,
a weight ratio between the hexose and the pentose contained in said fermentation feedstock is 1:9 to 9:1, and
a concentration of said pentose in a total amount of said filtrate is not more than 5 g/L, and
the product is (i) an alcohol selected from the group consisting of ethanol, 1,3-propanediol 1,3-butanediol, 2,3-butanediol, 1,4-butanediol, glycerol, butanol, isobutanol, 2-butanol, and isopropanol, (ii) an organic acid selected from the group consisting of acetic acid, lactic acid, adipic acid, pyruvic acid, succinic acid, malic acid, itaconic acid, and citric acid, or (iii) a free amino acid.

2. The method according to claim 1 , wherein the pentose is xylose and the hexose is glucose.

3. The method according to claim 1 , wherein the total sugar concentration in the fermentation feedstock is 60 g/L to 300 g/L.

Assignees

• Toray Industries

Inventors

• Kobayashi Koji
• Watanabe Shiomi
• Isobe Kyohei
• Sawai Kenji
• Na Kyungsu
• Hiramatsu Shingo
• Yamada Katsushige

Classifications

• C12P7/58

  Aldonic, ketoaldonic or saccharic acids (uronic acids C12P19/00) · CPC title

• C12P7/14

  Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12P7/065 Primary

  with microorganisms other than yeasts · CPC title