Compositions for saccharification of cellulosic material
US-2024018560-A1 · Jan 18, 2024 · US
Ionic liquid-tolerant cellulase enzymes
US10378000B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10378000-B2 |
| Application number | US-201715798351-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 30, 2017 |
| Priority date | Feb 3, 2014 |
| Publication date | Aug 13, 2019 |
| Grant date | Aug 13, 2019 |
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Abstract
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The present invention provides ionic liquid-tolerant cellulases and method of producing and using such cellulases. The cellulases of the invention are useful in saccharification reactions using ionic liquid treated biomass.
First claim
Opening claim text (preview).
What is claimed is: 1. A composition comprising (i) an isolated ionic liquid-tolerant cellulase having β-glucosidase activity and comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:4; and (ii) an ionic liquid, wherein the ionic liquid has an imidazolium cation. 2. The composition of claim 1 , wherein the ionic liquid-tolerant cellulase comprises an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:4. 3. The composition of claim 1 , further comprising a lignocellulosic biomass. 4. The composition of claim 1 , wherein the ionic liquid-tolerant cellulase comprises the amino acid sequence of SEQ ID NO:4. 5. A method of increasing the yield of soluble sugar from a lignocellulosic biomass, the method comprising incubating lignocellulosic biomass with a composition comprising an ionic liquid-tolerant cellulase and an ionic liquid having an imidazolium cation in an enzymatic hydrolysis reaction, wherein the ionic liquid-tolerant cellulase has β-glucosidase activity and comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:4. 6. The method of claim 5 , wherein the ionic liquid-tolerant cellulase has at least 98% sequence identity to the amino acid sequence of SEQ ID NO:4. 7. The method of claim 5 , wherein the lignocellulosic biomass is pretreated with ionic liquid. 8. The composition of claim 1 , wherein the ionic liquid is 1-ethyl-3-methyl-imidazolium acetate ([C 2 mim][OAc]). 9. The method of claim 5 , wherein the ionic liquid-tolerant cellulase comprises the amino acid sequence of SEQ ID NO:4. 10. The method of claim 5 , wherein the ionic liquid is [C 2 mim][OAc].
Assignees
Inventors
Classifications
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
- C12N9/2445Primary
Beta-glucosidase (3.2.1.21) · CPC title
Beta-glucosidase (3.2.1.21) · CPC title
produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title
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Frequently asked questions
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- What does patent US10378000B2 cover?
- The present invention provides ionic liquid-tolerant cellulases and method of producing and using such cellulases. The cellulases of the invention are useful in saccharification reactions using ionic liquid treated biomass.
- Who is the assignee on this patent?
- Univ California, Nat Tech & Eng Solutions Sandia Llc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/2445. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 13 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).