Process for extraction and separation of oxyresveratrol from Artocarpus lakoocha Roxb

Patent metadata

Field	Value
Publication number	US-10376840-B2
Application number	US-201515517439-A
Country	US
Kind code	B2
Filing date	Oct 7, 2015
Priority date	Oct 7, 2014
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

The present invention relates to a process for separation of oxyresveratrol molecule from the extracted solution of Artocarpus lakoocha Roxb. through membrane application. The product can be obtained in excellent yield upto 81% in case of extraction using water as solvent and can be separated from the extracted mixture upto 98% using indigenously developed nanofiltration membrane. Only the desired Trans isomer is obtained and no cis isomerization takes place during the extraction process.

First claim

We claim:

1. A process for extraction and separation of oxyresveratrol from heartwood of Artocarpus lakoocha Roxb., said process comprising the steps of:
(i) mixing Artocarpus lakoocha Roxb. Powder obtained from the heartwood with water at a temperature in the range of 30° C.-70° C. for 1 to 10 hours to extract a solution and filtering the solution to obtain a solution enriched in oxyresveratrol;
(ii) subjecting the solution enriched in oxyresveratrol obtained in step (i) to a membrane treatment in a two compartment membrane cell containing a nanofiltration membrane selected from an Alpha cyclodextrin-polysulfone composite nanofiltration membrane, a Beta cyclodextrin-polysulfone composite nanofiltration membrane or a Gamma cyclodextrin-polysulfone composite nanofiltration membrane by circulating through the nanofiltration membrane with a peristaltic pump at a flow rate in the range of 42.50 ml/min to 107.14 ml/min to obtain oxyresveratrol; and
(iii) collecting the oxyresveratrol obtained in step (ii) from feed side of the membrane;
wherein the trans membrane pressure across the membrane in step (ii) is maintained within the range of 7.2 psi to 21.75 psi.

2. The process as claimed in claim 1 , wherein the mixing of Artocarpus lakoocha Roxb. powder with water is done at 50° C.

3. The process as claimed in claim 1 , wherein the mixing of Artocarpus lakoocha Roxb. powder with water is done for 4 hours.

4. The process as claimed in claim 1 , wherein the nanofiltration membrane is a beta cyclodextrin-polysulfone composite nanofiltration membrane.

5. The process as claimed in claim 1 , wherein the two compartment membrane cell has a feed side and a permeate side with volumes of 150 ml and 120 ml respectively.

6. The process as claimed in claim 1 , wherein the trans membrane pressure across the membrane is maintained at 14.50 psi.

7. The process as claimed in claim 1 , wherein the membrane is cyclically backwashed with ethanol.

8. The process as claimed in claim 1 , wherein the oxyresveratrol obtained is a trans isomer of oxyresveratrol.

Assignees

• Council Scient Ind Res

Inventors

• Hazarika Swapnali
• Konwar Dilip
• Borah Hirokjyoti
• Borthakur Somiron
• Barkakati Pranab
• Bora Mohan Modon
• Das Ram Nath

Classifications

• B01D2321/04

  Backflushing · CPC title

• B01D71/06

  Organic material · CPC title

• B01D2321/168

  Use of other chemical agents · CPC title

• B01D61/027 Primary

  Nanofiltration · CPC title

• B01D71/68

  Polysulfones; Polyethersulfones · CPC title