Fungal endophytes for improved crop yields and protection from pests

Patent metadata

Field	Value
Publication number	US-10375966-B2
Application number	US-201715723133-A
Country	US
Kind code	B2
Filing date	Oct 2, 2017
Priority date	Nov 6, 2013
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

The invention provides a synthetic combination of a crop and at least one fungal endophyte, wherein the crop is a host plant of the endophyte. Provided are also methods and compositions for producing such synthetic combinations. The endophyte reproduces and enhances the agronomic characteristics of the crop. Methods for inoculating the host plant with the endophyte, for propagating the host-endophyte combination, and for detecting the presence of the endophyte and of its metabolites within a host plant are also described.

First claim

What is claimed is:

1. A method for improving drought resistance in a cotton plant, the method comprising:
contacting a cotton seed with a fluid seed coating comprising purified filamentous, spore-forming, facultative fungal endophytes of at least one Chaetomium species wherein the contacted seed contains at least 10 CFU up to 10,000 CFU of the facultative fungal endophyte coated onto the surface of the seed, wherein the facultative fungal endophytes are present in the fluid seed coating in an amount effective to improve drought resistance of a cotton plant grown from the contacted seed compared to a reference cotton plant grown from a reference seed.

2. The method of claim 1 , wherein the fluid seed coating contains at least 100 (10^2) spores/ml or 100,000 (10^4) spores/g dry weight of the facultative fungal endophytes.

3. The method of claim 1 , wherein the facultative fungal endophytes are present in the fluid seed coating in an amount effective to increase biomass.

4. The method of claim 1 , wherein the facultative fungal endophytes are species Chaetomium globosum.

5. The method of claim 4 , wherein the cotton plant is variety LA122.

6. The method of claim 1 , wherein the cotton plant is variety LA122.

7. The method of claim 1 , wherein the facultative fungal endophytes are present in the fluid seed coating in an amount effective to reduce yield loss.

8. The method of claim 7 , wherein yield loss is reduced by at least 5%.

9. The method of claim 7 , wherein the reduction in yield loss results in a yield increase of at least 5%.

10. The method of claim 1 , wherein enhanced resistance to drought stress is assessed by withholding water from 7-day old seedlings of the cotton plant grown in the greenhouse, wherein the seedlings have increased time to wilt as compared to a seedling grown from a reference seed.

11. The method of claim 1 , wherein the seed is surface sterilized before contacting the seed with the fluid seed coating.

12. The method of claim 1 , wherein the contacted seed contains at least 100 CFU, at least 300 CFU, at least 1,000 CFU, at least 3,000 CFU, or at least 10,000 CFU of the facultative fungal endophytes coated onto the surface of the seed.

Assignees

• Texas A & M Univ Sys

Inventors

• Sword Gregory A

Classifications

• A01N63/30 Primary

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• A01H5/10

  Seeds · CPC title

• A01N63/04 Primary

  Human Necessities · mapped topic

• A01N65/00

  Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title