Microbial compositions and methods of use for benefiting plant growth and treating plant disease

Patent metadata

Field	Value
Publication number	US-10375964-B2
Application number	US-201515539991-A
Country	US
Kind code	B2
Filing date	Dec 28, 2015
Priority date	Dec 29, 2014
Publication date	Aug 13, 2019
Grant date	Aug 13, 2019

Abstract

Compositions and methods are provided for a combination of a new strain of Bacillus amyloliquefaciens RTI301 and a new strain of Bacillus subtilis RTI477, the combination having growth promoting activity and activity against plant pathogens. The compositions containing the RTI301 and RTI477 strains are useful for benefiting plant growth and/or conferring protection against a pathogenic infection when applied to plant roots, seeds, callus tissue, grafts, and cuttings. Synergistic results are observed for the combination of the strains, and the combination of strains is useful to increase yield in crops including soybean and corn. The compositions containing the combination of strains can be applied alone or in combination with other microbial, biological, or chemical insecticides, fungicides, nematicides, bacteriocides, herbicides, plant extracts, plant growth regulators, and fertilizers.

First claim

That which is claimed:

1. A method for benefiting plant growth and/or plant health, the method comprising:
delivering to seed of a plant, roots of a plant, or soil surrounding a plant a composition comprising:
a biologically pure culture of a Bacillus velezensis RTI301 deposited as ATCC No. PTA-121165, or a mutant thereof having all the identifying characteristics thereof; and
a biologically pure culture of a Bacillus subtilis RTI477 deposited as ATCC No. PTA-121167, or a mutant thereof having all the identifying characteristics thereof, wherein delivery of the composition benefits plant growth and/or plant health.

2. The method of claim 1 , wherein the benefits to plant growth and/or plant health comprise increased yield, improved seedling vigor, improved root development, improved plant growth, improved plant health, improved appearance, improved resistance to plant pathogens, reduced pathogenic infection, or a combination thereof.

3. The method of claim 2 , wherein the plant pathogen comprises one or a combination of insects, nematodes, plant pathogenic fungi, or plant pathogenic bacteria, or
wherein the plant pathogen comprises one or more of a plant fungal pathogen, a plant bacterial pathogen, a rust fungus, a Botrytis spp., a Botrytis cinerea , a Botrytis squamosa , an Erwinia spp., an Erwinia carotovora , an Erwinia amylovora , a Dickeya spp., a Dickeya dadantii , a Dickeya solani , an Agrobacterium spp., a Agrobacterium tumefaciens , a Xanthomonas spp., a Xanthomonas axonopodis, a Xanthomonas campestris pv. carotae , a Xanthomonas pruni , a Xanthomonas arboricola , a Xanthomonas oryzae pv. oryzae , a Xylella spp., a Xylella fastidiosa , a Candidatus spp., a Candidatus liberibacter , a Fusarium spp., a Fusarium colmorum , a Fusarium graminearum , a Fusarium oxysporum , a Fusarium oxysporum f sp. Cubense , a Fusarium oxysporum f sp. Lycopersici , a Fusarium virguliforme , a Sclerotinia spp., a Sclerotinia sclerotiorum , a Sclerotinia minor, Sclerotinia homeocarpa , a Cercospora/Cercosporidium spp., an Uncinula spp., an Uncinula necator (Powdery Mildew), a Podosphaera spp. (Powdery Mildew), a Podosphaera leucotricha , a Podosphaera clandestine , a Phomopsis spp., a Phomopsis viticola , an Alternaria spp., an Alternaria tenuissima , an Alternaria porri , an Alternaria alternate, an Alternaria solani , an Alternaria tenuis , a ispp., a Pseudomonas syringae pv. Tomato, a Phytophthora spp., a Phytophthora infestans , a Phytophthora parasitica , a Phytophthora sojae , a Phytophthora capsici , a Phytophthora cinnamon , a Phytophthora fragariae , a Phytophthora spp., a Phytophthora ramorum , a Phytophthora palmivara , a Phytophthora nicotianae , a Phakopsora spp., a Phakopsora pachyrhizi , a Phakopsora meibomiae , an Aspergillus spp., an Aspergillus flavus , an Aspergillus niger , a Uromyces spp., a Uromyces appendiculatus , a Cladosporium spp., a Cladosporium herbarum , a Rhizopus spp., a Rhizopus arrhizus , a Penicillium spp., a Rhizoctonia spp., a Rhizoctonia solani , a Rhizoctonia zeae , a Rhizoctonia oryzae , a Rhizoctonia caritae , a Rhizoctonia cerealis , a Rhizoctonia crocorum , a Rhizoctonia fragariae , a Rhizoctonia ramicola , a Rhizoctonia rubi , a Rhizoctonia leguminicola , a Macrophomina phaseolina , a Magnaorthe oryzae , a Mycosphaerella spp., Mycosphaerella graminocola, a Mycosphaerella fijiensis (Black sigatoga), a Mycosphaerella pomi , a Mycosphaerella citri , a Magnaporthe spp., a Magnaporthe grisea , a Monilinia spp., a Monilinia fruticola , a Monilinia vacciniicorymbosi , a Monilinia laxa , a Colletotrichum spp., a Colletotrichum gloeosporiodes , a Colletotrichum acutatum , a Colletotrichum Candidum , a Diaporthe spp., a Diaporthe citri , a Corynespora spp., a Corynespora Cassiicola , a Gymnosporangium spp., a Gymnosporangium juniperi - virginianae , a Schizothyrium spp., a Schizothyrium pomi , a Gloeodes spp., a Gloeodes pomigena , a Botryosphaeria spp., a Botryosphaeria dothidea , a Neofabraea spp., a Wilsonomyces spp., a Wilsonomyces carpophilus , a Sphaerotheca spp., a Sphaerotheca macularis , a Sphaerotheca pannosa , a Erysiphe spp., a Stagonospora spp., a Stagonospora nodorum , a Pythium spp., a Pythium ultimum , a Pythium aphanidermatum , a Pythium irregularum , a Pythium ulosum , a Pythium lutriarium , a Pythium sylvatium , a Venturia spp, a Venturia inaequalis , a Verticillium spp., a Ustilago spp., a Ustilago nuda , a Ustilago maydis , a Ustilago scitaminea , a Claviceps spp., a Claviceps puprrea , a Tilletia spp., a Tilletia tritici , a Tilletia laevis , a Tilletia horrid , a Tilletia controversa , a Phoma spp., a Phoma glycinicola , a Phoma exigua , a Phoma lingam , a Cocliobolus sativus , a Gaeumanomyces gaminis , a Colleototricum spp., a Rhychosporium spp., Rhychosporium secalis , a Biopolaris spp., a Helminthosporium spp., a Helminthosporium secalis , a Helminthosporium maydis , a Helminthosporium solai , or a Helminthosporium tritici - repentis.

4. The method of claim 1 , wherein the composition is in the form of a liquid, an oil dispersion, a dust, a dry wettable powder, a spreadable granule, or a dry wettable granule, or
wherein the composition is in the form of a liquid and each of the Bacillus subtilis RTI477 and the Bacillus velezensis RTI301 are present at a concentration of from about 1.0×10 8 CFU/ml to about 1.0×10 12 CFU/ml, or
wherein the composition is in the form of a dust, a dry wettable powder, a spreadable granule, or a dry wettable granule and each of the Bacillus subtilis RTI477 and the Bacillus velezensis RTI301 are present in an amount of from about 1.0×10 8 CFU/g to about 1.0×10 12 CFU/g, or
wherein the composition is in the form of an oil dispersion and each of the Bacillus subtilis RTI477 and the Bacillus velezensis RTI301 are present at a concentration of from about 1.0×10 8 CFU/ml to about 1.0×10 12 CFU/ml, or
wherein the Bacillus subtilis RTI477 and the Bacillus velezensis RTI301 are present in the form of spores or vegetative cells.

5. The method of claim 1 , wherein the composition further comprises one or a combination of a carrier, a dispersant or a yeast extract.

6. The method of claim 1 , wherein the plant comprises monocots, dicots, Cereals, Corn, Sweet Corn, Popcorn, Seed Corn, Silage Corn, Field Corn, Rice, Wheat, Barley, Sorghum, Asparagus, Berry, Blueberry, Blackberry, Raspberry, Loganberry, Huckleberry, Cranberry, Gooseberry, Elderberry, Currant, Caneberry, Bushberry, Brassica Vegetables, Broccoli, Cabbage, Cauliflower, Brussels Sprouts, Collards, Kale, Mustard Greens, Kohlrabi, Cucurbit Vegetables, Cucumber, Cantaloupe, Melon, Muskmelon, Squash, Watermelon, Pumpkin, Eggplant, Bulb Vegetables, Onion, Garlic, Shallots, Citrus, Orange, Grapefruit, Lemon, Tangerine, Tangelo, Pummelo, Fruiting Vegetables, Pepper, Tomato, Ground Cherry, Tomatillo, Okra, Grape, Herbs/Spices, Leafy Vegetables, Lettuce, Celery, Spinach, Parsley, Radicchio, Legumes/Vegetables (succulent and dried beans and peas), Beans, Green beans, Snap beans, Shell beans, Soybeans, Dry Beans, Garbanzo beans, Lima beans, Peas, Chick peas, Split peas, Lentils, Oil Seed Crops, Canola, Castor, Coconut, Cotton, Flax, Oil Palm, Olive, Peanut, Rapeseed, Safflower, Sesame, Sunflower, Soybean, Pome Fruit, Apple, Crabapple, Pear, Quince, Mayhaw, Root/Tuber and Corn Vegetables, Carrot, Potato, Sweet Potato, Cassave, Beets, Ginger, Horseradish, Radish, Ginseng, Turnip, Stone Fruit, Apricot, Cherry, Nectarine, Peach, Plum, Prune, Strawberry, Tree Nuts, Almond, Pistachio, Peca n, Walnut, Filberts, Chestnut, Cashew, Beechnut, Butternut, Macadamia, Kiwi, Ban

Assignees

• Fmc Corp

Inventors

• Taghavi Safiyh
• Van Der Lelie Daniel
• Mcleod Roderick
• Brost Kevin R
• Kibbee John E

Classifications

• A01N53/00

  Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12R1/125

  Chemistry & Metallurgy · mapped topic

• A01N2300/00

  Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48 · CPC title

• C12R1/07

  Chemistry & Metallurgy · mapped topic