Clot protection and detection algorithm for activated clotting time testing

Patent metadata

Field	Value
Publication number	US-10371694-B2
Application number	US-201615135327-A
Country	US
Kind code	B2
Filing date	Apr 21, 2016
Priority date	Dec 6, 2011
Publication date	Aug 6, 2019
Grant date	Aug 6, 2019

Abstract

Activated clotting time (ACT) tests detect blood clotting time based on the viscosity changes of a test sample, using a ferromagnetic washer lifted to the top of a test chamber and then dropped from the top via gravity; a drop time greater than a preset threshold value indicates clotting of the test sample. Blood samples which have high levels of heparin usually produce very weak clots that may easily be destroyed by the lifting movement of the washer. But if the clot threshold is set low to detect the weak clots, false detections occur during early testing cycles when activators are not fully suspended during the mixing cycle. Improved algorithms for lifting the washer and adjusting over time enable accurate detection of weak clots.

First claim

What is claimed is:

1. A method of detecting formation of a clot in a sample of blood with a ferromagnetic washer moving over a distance through the sample, comprising:
a. determining calibration values for at least one of a distance span and a drop velocity of the ferromagnetic washer moving through the sample when the blood is unclotted;
b. measuring the distance span or the drop velocity of the ferromagnetic washer moving through the sample; and
c. detecting formation of the clot by comparing the value of the distance span or drop velocity measured with the corresponding calibration value.

2. The method of claim 1 , wherein:
measuring the distance span or the drop velocity of the ferromagnetic washer moving through the sample is repeated in a plurality of consecutive washer cycles; and
detecting formation of the clot is performed in each of the plurality of consecutive washer cycles.

3. The method of claim 1 , in which a calibration value for at least one of a distance span and a drop velocity of the ferromagnetic washer moving through the sample when the blood is unclotted is determined from at least a first pass of the ferromagnetic washer moving through the sample in which only a portion of distance through the sample moved by the ferromagnetic washer is considered, and a second pass in which a fixed time based on only the portion of the distance considered in the first pass is determined.

Assignees

• Medtronic Inc

Inventors

• Cheek Daniel
• Erickson Lawrence
• Huang Trevor
• Kanayinkal Tessy
• Petersen Craig
• Yuan Charlene

Classifications

• G01N33/4905 Primary

  Determining clotting time of blood (by chemical methods G01N33/86, C12Q1/56) · CPC title

• G01N33/86

  involving blood coagulating time {or factors, or their receptors} · CPC title