Generation of transgenic canola with low or no saturated fatty acids

Patent metadata

Field	Value
Publication number	US-10370674-B2
Application number	US-201715398481-A
Country	US
Kind code	B2
Filing date	Jan 4, 2017
Priority date	Oct 8, 2004
Publication date	Aug 6, 2019
Grant date	Aug 6, 2019

Abstract

Compositions and methods include genetically encoding and expressing a novel delta-9 desaturase in plant cells. In some embodiments, methods of expressing nucleic acids in a plant cell to take advantage of the delta-9 desaturase enzyme's activity, such that the percent composition of saturated fatty acids in plant seeds is decreased and there is a concomitant increase in Δ9 fatty acids. In other embodiments, amino acid sequences have delta-9 desaturase activity. Methods can involve expression of delta-9 desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of mono unsaturated fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

First claim

What is claimed is:

1. A method for decreasing the amount of saturated fatty acids in oil from a plant, the method comprising:
transforming plant material with a nucleic acid molecule comprising a heterologous gene regulatory element operably linked to a polynucleotide encoding a delta-9 desaturase enzyme comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:1,
wherein the plant material also comprises an extraplastidial desaturase selected from the group consisting of LnD9DS desaturase, HzD9DS desaturase, and MgD9DS desaturase;
culturing the transformed plant material to obtain a plant; and
extracting oil from the plant.

2. The method of claim 1 , wherein the plant material is a seed.

3. The method of claim 1 , wherein the plant is selected from a genus selected from the group consisting of Arabidopsis, Borago, Canola, Ricinus, Theobroma, Zea, Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes , Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis , rapeseed, Carthamus, Glycine, Soja, Hehanthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale , and the other members of the Gramineae.

Assignees

• Dow Agrosciences Llc

Inventors

• Gachotte Daniel J
• Merlo Ann Owens
• Thompson Mark A
• Walsh Terence A
• Wilson Beth Rubin
• Welter Mary

Classifications

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12Y114/99

  Miscellaneous (1.14.99) · CPC title

• C12N9/0083

  Miscellaneous (1.14.99) · CPC title

• C12Y114/00

  Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) · CPC title

• C12Y114/19001

  Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase · CPC title