Tricyclic mosquitocides and methods of making and using thereof

Patent metadata

Field	Value
Publication number	US-10370385-B2
Application number	US-201515325320-A
Country	US
Kind code	B2
Filing date	Jul 10, 2015
Priority date	Jul 10, 2014
Publication date	Aug 6, 2019
Grant date	Aug 6, 2019

Abstract

Compounds that inhibit digestion in blood-ingesting pests are described herein. In one embodiment, the compounds described herein block entry of blood into the midgut and thereby inhibit digestion and nutrient processing. In another embodiment, the compounds described herein prevent pathogens contained in the blood meal from entering the midgut where they could cross the epithelial cell layer and infect the mosquito. The compounds can be administered to a population of blood-ingesting pests, such as mosquitos, directly or indirectly in an effective amount to prevent mosquitoes from transmitting diseases such as malaria, dengue fever, West Nile virus and lymphatic filariasis. Preferably, the compounds are lethal to blood-ingesting pests. The compounds can be combined with one or more excipients to prepare compositions.

First claim

We claim:

1. A composition comprising a compound having the formula:

wherein
X is O, S, CR 10 R 11 , SiR 10 R 11 , or NR 10 ;
Y is O, S, CR 10 R 11 ;
Z is O, S, CR 10 R 11 ;
R 1 -R 11 are each independently selected from the group consisting of hydrogen, hydroxyl, cyano, nitro, halogen, NH 2 , SH, SR 12 , OR 12 , N(R 12 ) 2 , OCON(R 12 ) 2 , C(═O)R 12 , OC(═O)R 12 , C(═O)OR 12 , COOH, COO − M + , CONH 2 , CON(R 12 ) 2 , C(═O)H, substituted or unsubstituted linear, branched, or cyclic alkyl, alkenyl, or alkynyl; substituted or unsubstituted linear, branched, or cyclic heteroalkyl, heteroalkenyl, or heteroalkynyl, substituted or unsubstituted aryl or heteroaryl, SOR 12 , SOOR 12 , SO 3 R 12 , SO 4 R 12 , NR 12 COR 13 , phosphoryl, sulfamoyl, and sulfonamido; wherein each occurrence of R 12 or R 13 is selected from the group consisting of hydrogen, substituted or unsubstituted linear, branched, or cyclic alkyl, alkenyl, or alkynyl; substituted or unsubstituted linear, branched, or cyclic heteroalkyl, heteroalkenyl, or heteroalkynyl, and substituted or unsubstituted aryl or heteroaryl;
wherein the dotted line represents a bond that is either present or absent;
and
wherein the compound is in an effective amount to inhibit digestion or nutrient processing in at least 40% of a population of blood-ingesting pests, reduce follicle size in female blood-ingesting pests compared to the follicle size when the pests are not exposed to the compound, or provide a lethal concentration against pest larvae, or a combination thereof, and
wherein the compound is in a concentration of less than 0.25 mM.

2. The composition of claim 1 , wherein the compound has the following stereochemistry

3. The composition of claim 1 , wherein
X is sulfur;
Y is oxygen;
Z is oxygen; or
X is sulfur and Y and Z are both oxygen.

4. The composition of claim 1 , wherein R 1 is a substituted or unsubstituted alkyl.

5. The compound of claim 4 , wherein
R 1 is methyl;
R 2 is hydrogen;
R 3 is hydrogen;
R 6 is hydrogen;
R 7 is hydrogen; or
R 4 and R 5 are hydrogen.

6. The composition of claim 1 , wherein R 8 is a substituted or unsubstituted aryl.

7. The composition of claim 6 , wherein R 8 is a substituted or unsubstituted phenyl.

8. The composition of claim 7 , wherein R 8 is an unsubstituted phenyl.

9. The composition of claim 1 , wherein the compound is:

10. The composition of claim 1 , wherein the compound has the formula:

11. The composition of claim 1 , in the form of a liquid is selected from the group consisting of a solution, suspension, dispersion, and an emulsion.

12. The composition of claim 1 , wherein the effective amount of the compound is non-toxic to plants, animals, and/or arthropods that do not function as vectors of human pathogens.

13. A method of preventing the spread of mosquito-borne illnesses, comprising administering to a site or region containing one or more mosquitoes an effective amount of a composition comprising a compound having the formula:

wherein
X is O, S, CR 10 R 11 , SiR 10 R 11 , or NR 10 ;
Y is O, S, CR 10 R 11 ;
Z is O, S, CR 10 R 11 ;
R 1 -R 11 are each independently selected from the group consisting of hydrogen, hydroxyl, cyano, nitro, halogen, NH 2 , SH, SR 12 , OR 12 , N(R 12 ) 2 , OCON(R 12 ) 2 , C(═O)R 12 , OC(═O)R 12 , C(═O)OR 12 , COOH, COO − M + , CONH 2 , CON(R 12 ) 2 , C(═O)H, substituted or unsubstituted linear, branched, or cyclic alkyl, alkenyl, or alkynyl; substituted or unsubstituted linear, branched, or cyclic heteroalkyl, heteroalkenyl, or heteroalkynyl, substituted or unsubstituted aryl or heteroaryl, SOR 12 , SOOR 12 , SO 3 R 12 , SO 4 R 12 , NR 12 COR 13 , phosphoryl, sulfamoyl, and sulfonamido; wherein each occurrence of R 12 or R 13 is selected from the group consisting of hydrogen, substituted or unsubstituted linear, branched, or cyclic alkyl, alkenyl, or alkynyl; substituted or unsubstituted linear, branched, or cyclic heteroalkyl, heteroalkenyl, or heteroalkynyl, and substituted or unsubstituted aryl or heteroaryl;
wherein the dotted line represents a bond that is either present or absent; and
wherein the compound is in an effective amount to inhibit digestion or nutrient processing in at least 40% of a population of blood-ingesting pests, reduce follicle size in female blood-ingesting pests compared to the follicle size when the pests are not exposed to the compound, or provide a lethal concentration against pest larvae, or a combination thereof, and
wherein the compound is in a concentration of less than 0.25 mM.

14. The method of claim 13 , wherein the site or region is a body of water or contains standing water.

15. The method of claim 13 , wherein the compound is:

16. The composition of claim 1 , wherein the composition formed by introducing pellets comprising the compound into a continuous aqueous phase.

17. The composition of claim 1 , wherein the composition formed by introducing flakes comprising the compound into a continuous aqueous phase.

18. The composition of claim 1 , wherein the composition is in the form of a liquid, and wherein the compound is dispersed or dissolved in an aqueous continuous phase.

19. The composition of claim 1 , further comprising a wetting agent, surfactant, or carrier, or a combination thereof.

20. The compound of claim 19 , wherein the composition comprises a surfactant, and wherein the surfactant is an anionic, cationic, amphoteric, or nonionic surface-active agent.

Assignees

• Univ Arizona

Inventors

• Njardarson Jon
• Isoe Jun
• Miesfeld Roger

Classifications

• C07D495/04 Primary

  Ortho-condensed systems · CPC title

• A01N43/90

  having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system · CPC title

• Y02A50/30

  Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change · CPC title