Segmentable container and method of segmenting substance contained in container

Patent metadata

Field	Value
Publication number	US-10369563-B2
Application number	US-201715456434-A
Country	US
Kind code	B2
Filing date	Mar 10, 2017
Priority date	Mar 30, 2012
Publication date	Aug 6, 2019
Grant date	Aug 6, 2019

Abstract

Provided are a simple, quick, and low-processing cost method capable of reliably separating part of an accommodated substance accommodated in a vessel only by simple mechanical operation while maintaining a hermetically-sealed state without any contact with an outside atmosphere, as well as a vessel with which the method may be used. A separable vessel comprises a first vessel portion, a second vessel portion which is contiguous with the first vessel portion, a fracture-inducing portion formed between the first vessel portion and the second vessel portion, and a self-fusing material provided on an outer surface so as to cover the fracture-inducing portion; and may contain an accommodated substance therein.

First claim

The invention claimed is:

1. A manipulation device for manipulating an object component in a vessel, comprising:
the vessel which is a separable vessel comprising: a fracture-inducing portion formed in a position where fracture should be caused to separate the vessel into a first vessel portion and a second vessel portion; and a self-fusing material provided on an outer surface so as to cover the fracture-inducing portion, and the vessel containing an accommodated substance therein;
magnetic particles that should capture and transport an object component; and
a magnetic field application member for applying a magnetic field to the vessel so that the magnetic particles can be moved from an inside of the first vessel portion to an inside of the second vessel portion,
wherein the fracture-inducing portion has a reduced wall thickness and/or a reduced material strength as compared with the rest of the vessel; and
wherein the magnetic field application member is a permanent magnet or an electromagnet.

2. The manipulation device according to claim 1 , the vessel being a manipulation vessel for subjecting a sample containing an object component to a predetermined manipulation therein, wherein
the first vessel portion is a manipulation portion for subjecting a sample containing an object component to a predetermined manipulation,
the second vessel portion is a collection portion for collecting a target substance from the manipulation portion, and
the accommodated substance is a manipulation medium selected from the group consisting of a liquid, a solid, a gas, and a dispersion system, as a field for performing manipulation to which the object component is to be subjected.

3. The manipulation device according to claim 2 , wherein the manipulation portion comprises a column for chromatography, and the manipulation medium comprises a filling material for chromatography and a developing solvent.

4. The manipulation device according to claim 2 , the vessel having a tubular shape, wherein the manipulation medium is a multi-layered object in which layers of an aqueous liquid and a gel are alternately stacked in a longitudinal direction.

5. The manipulation device according to claim 2 , wherein the manipulation medium comprises a droplet encapsulating medium and an encapsulated aqueous droplet.

6. The manipulation device according to claim 1 , wherein the vessel further comprises a protective member on an outer surface of the self-fusing material.

7. The manipulation device according to claim 1 , wherein the self-fusing material has a thickness of 0.01 to 5 mm per 1 cm 2 of area of a plane within an outer periphery of the vessel.

8. The manipulation device according to claim 1 , wherein the self-fusing material is selected from the group consisting of isobutylene-isoprene copolymers, ethylene-propylene-diene copolymers, polyisobutylene, paraffin, polyvinyl acetate, polyurethane, polydimethyl siloxane, ethylene propylene copolymers, hydrogel polymers, (meth)acrylic acid ester copolymers, silicone rubber, and natural rubber.

9. The manipulation device according to claim 1 , wherein the self-fusing material is a thermoplastic resin having a glass transition temperature of 50° C. to 180° C.

10. The manipulation device according to claim 9 , wherein the thermoplastic resin is selected from the group consisting of polyethylene, polypropylene, polystyrene, ethylene-vinyl acetate copolymers, polyacetal, polymethyl methacrylate, polyvinyl alcohol, polyvinyl chloride, vinyl chloride-vinyl acetate copolymers, vinyl chloride-acrylic acid ester copolymers, polyvinylidene chloride, and vinylidene chloride-acrylic acid ester copolymers.

Assignees

• Shimadzu Corp

Inventors

• Ohashi Tetsuo

Classifications

• B01L3/502784

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L3/52

  Containers specially adapted for storing or dispensing a reagent (B01L3/02 takes precedence; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002) · CPC title

• B01L3/502 Primary

  with fluid transport, e.g. in multi-compartment structures · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• Y10T156/1005

  by inward collapsing of portion of hollow body · CPC title