Immunostimulator and method for producing the same

Patent metadata

Field	Value
Publication number	US-10369218-B2
Application number	US-201615387951-A
Country	US
Kind code	B2
Filing date	Dec 22, 2016
Priority date	Dec 25, 2015
Publication date	Aug 6, 2019
Grant date	Aug 6, 2019

Abstract

Disclosed is an immunostimulator containing virus-like particles, in which the virus-like particles contain an outer coat protein containing an amino acid sequence selected from the group consisting of SEQ ID No. 1, SEQ ID No. 26, SEQ ID No. 33, SEQ ID No. 35, SEQ ID No. 37, SEQ ID No. 39, SEQ ID No. 41, SEQ ID No. 43 and SEQ ID No. 45; the outer coat protein constitutes an outer coat of the virus-like particles; and the virus-like particles do not substantially contain a genome DNA of SV40.

First claim

What is claimed is:

1. A method for potentiating an immune effect of a living body, comprising administering an adjuvant and a vaccine comprising an exogenous antigen to a living body and inducing production of CD86+ lymphocytes in the living body, the adjuvant comprising a pharmacologically effective amount of virus-like particles,
wherein the virus-like particles comprise an outer coat protein comprising the amino acid sequence of SEQ ID NO: 1;
wherein the outer coat protein constitutes an outer coat of the virus-like particles;
wherein the virus-like particles do not substantially comprise a genome DNA of SV40; and
wherein the virus-like particles do not substantially comprise the exogenous antigen.

2. The method according to claim 1 , wherein the virus-like particles do not substantially comprise VP2, VP3, or both of them.

3. The method according to claim 1 , wherein the administrating step is conducted by oral administration, transmucosal administration, parenteral administration, or transdermal administration.

4. A method for potentiating an immune effect of a living body, comprising administering an adjuvant and a vaccine comprising an exogenous antigen to a living body, the adjuvant comprising a pharmacologically effective amount of virus-like particles;
wherein the virus-like particles comprise an outer coat protein comprising the amino acid sequence of SEQ ID NO: 1;
wherein the virus-like particles do not substantially comprise a genome DNA of SV40; and
wherein the virus-like particles do not substantially comprise the exogenous antigen.

Assignees

• Univ Saitama Medical
• Sysmex Corp

Inventors

• Handa Hiroshi
• Kawano Masaaki
• Kato Masahiko

Classifications

• C12N2710/14043

  viral genome or elements thereof as genetic vectore · CPC title

• A61K2039/555

  characterised by a specific combination antigen/adjuvant · CPC title

• A61P37/04

  Immunostimulants · CPC title

• C12N2710/22023

  Virus like particles [VLP] · CPC title

• A61K39/39 Primary

  characterised by the immunostimulating additives, e.g. chemical adjuvants · CPC title