Method for treating biological samples, especially food samples

Patent metadata

Field	Value
Publication number	US-10365191-B2
Application number	US-201515521567-A
Country	US
Kind code	B2
Filing date	Oct 23, 2015
Priority date	Oct 24, 2014
Publication date	Jul 30, 2019
Grant date	Jul 30, 2019

Abstract

A method for treating a biological sample, preferably a food sample which may contain one or more species of interest, including a step of decomplexification by acoustophoresis.

First claim

The invention claimed is:

1. A method for treating a food sample which may contain one or more microorganisms of interest and non-specific particles, the treatment method comprising a step of decomplexification, wherein the decomplexification comprises:
a) introducing all or part of the food sample into a first inlet orifice of an acoustophoresis device,
b) introducing a buffer solution into a second inlet orifice of the acoustophoresis device, the inlet orifices being fluidically connected to at least two outlet orifices by a separation channel,
c) introducing the buffer and the food sample at respective flow rates capable of generating a laminar flow in the separation channel, and
d) carrying out a step of separation of the microorganisms and the nonspecific particles in the food sample by acoustophoresis so as to promote the concentration of the non-specific particles present in the food sample in at least one of the outlet orifices of the acoustophoresis device, wherein the method further comprises:
e) a step of carrying out an enrichment of the microorganisms of interest in the food sample before the introduction of the food sample into the acoustophoresis device, and
f) a step of lysing the microorganisms of interest following the separation step.

2. The treatment method as claimed in claim 1 , further comprising a step of counting the total microorganisms present in the food sample following the separation step and before the lysis step.

3. The treatment method as claimed in claim 1 , further comprising a step of specific or non-specific capture of the one or more microorganisms of interest on a capture support followed by a step of concentration by immunological separation.

4. The treatment method as claimed in claim 1 , further comprising a step of amplification and analysis of the lysed sample.

5. The treatment method as claimed in claim 1 , further comprising a step of analysis of the one or more captured microorganisms of interest.

6. The treatment method as claimed in claim 1 , further comprising a step of labeling with a fluorescent label the one or more microorganisms of interest, the fluorescent label being specific to the microorganisms of interest, following the step of separation by acoustophoresis.

7. The treatment method as claimed in claim 6 , further comprising a step of analysis by flow cytometry aimed at detecting the presence of the fluorescent label.

8. The treatment method as claimed in claim 1 , the food sample being prefiltered through a filter or a membrane before the introduction of the food sample into the acoustophoresis device.

9. The treatment method as claimed in claim 1 , the food sample being homogenized before the introduction of the biological sample into the acoustophoresis device, the introduction being carried out without prior filtration step.

10. The treatment method as claimed in claim 1 , further comprising carrying out a second step of separation by acoustophoresis of the food sample by reintroduction of the sample into the separation channel of the device or by introduction of the sample into a second channel of separation by acoustophoresis.

11. The treatment method as claimed in claim 10 , the second step of separation by acoustophoresis being carried out with a buffer different than the first step.

12. The treatment method as claimed in claim 3 , wherein the specific capture of the one or more microorganisms of interest is implemented on a magnetic capture support.

13. The treatment method as claimed in claim 4 , wherein the amplification and analysis of the lysed sample are implemented by quantitative PCR.

14. The treatment method as claimed in claim 5 , wherein the analysis of the one or more captured microorganisms of interest is by means of one or more immunoassays specific for the one or more captured microorganisms of interest.

Assignees

• Biomerieux Sa

Inventors

• Broyer Patrick
• Patel Pradip
• Pamme Nicole
• Raymond Jean-Claude

Classifications

• G01N2015/142

  Acoustic or ultrasonic focussing · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N2001/4094

  using ultrasound · CPC title

• G01N2001/4088

  filtration · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title