Pesticidal genes and methods of use

Patent metadata

Field	Value
Publication number	US-10364439-B2
Application number	US-201615158380-A
Country	US
Kind code	B2
Filing date	May 18, 2016
Priority date	Jun 3, 2015
Publication date	Jul 30, 2019
Grant date	Jul 30, 2019

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

First claim

That which is claimed is:

1. A recombinant polypeptide having pesticidal activity, said polypeptide comprising
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:79, wherein said polypeptide of (a) has pesticidal activity and further comprises a heterologous amino acid sequence chemically linked to said polypeptide of (a), wherein said polypeptide is pesticidal against at least one of Lepidopteran or Coleopteran pests; or
(b) a polypeptide comprising the amino acid sequence of SEQ ID NO:79, wherein said polypeptide of (b) further comprises a heterologous amino acid sequence chemically linked to said polypeptide of (b).

2. A composition comprising the polypeptide of claim 1 .

3. A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a nucleic acid sequence encoding an amino acid sequence comprising
(a) at least 95% sequence identity to the amino acid sequence of SEQ ID NO:79, wherein said polypeptide has pesticidal activity against at least one of Lepidopteran or Coleopteran pests; or
(b) the amino acid sequence of SEQ ID NO:79.

4. The recombinant nucleic acid of claim 3 , wherein said nucleic acid molecule is a synthetic sequence designed for expression in a plant.

5. The recombinant nucleic acid molecule of claim 3 , wherein said heterologous promoter capable of directing expression in a plant cell.

6. The recombinant nucleic acid molecule of claim 3 , wherein said-heterologous promoter capable of directing expression in a bacteria.

7. A host cell comprising the recombinant nucleic acid molecule of claim 3 .

8. The host cell of claim 7 , wherein said host cell is a bacterial host cell.

9. A vector comprising the recombinant nucleic acid molecule of claim 3 .

10. A host cell comprising the vector of claim 9 .

11. A composition comprising the host cell of claim 10 .

12. The composition of claim 2 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.

13. The composition of claim 12 , wherein said composition comprises from about 1% to about 99% by weight of said polypeptide.

14. A method for controlling a pest population, said method comprising contacting said pest population with a pesticidal-effective amount of the composition of claim 2 .

15. A method for producing a polypeptide with pesticidal activity, said method comprising culturing the host cell of claim 10 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

16. A plant having stably incorporated into its genome a DNA construct comprising:
(a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:79; or
(b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:79, wherein said polypeptide has pesticidal activity against at least one of Lepidopteran or Coleopteran pests.

17. A transgenic seed of the plant of claim 16 .

18. A method for protecting a plant from an insect pest, said method comprising growing a plant or seed thereof that expresses
(a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:79; or
(b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:79, wherein said polypeptide has pesticidal activity against at least one of Lepidopteran or Coleopteran pests.

19. A method for increasing yield in a plant, said method comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in the plant operably linked to a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence comprises
(a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:79; or
(b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:79, wherein said polypeptide has pesticidal activity against at least one of Lepidopteran or Coleopteran pests.

Assignees

• Agbiome Inc

Inventors

• Parks Jessica
• Roberts Kira Bulazel
• Thayer Rebecca E

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C12N15/8285

  for nematode resistance · CPC title

• Y02A40/162

  Cross-Sectional Technologies · mapped topic

• A01N37/46

  N-acyl derivatives · CPC title