Lactobacillus paracasei strain
US-12152232-B2 · Nov 26, 2024 · US
US10364415B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10364415-B2 |
| Application number | US-201515517146-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 7, 2015 |
| Priority date | Oct 9, 2014 |
| Publication date | Jul 30, 2019 |
| Grant date | Jul 30, 2019 |
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To provide an effective 1,4-dioxane-degrading bacteria culture method. Provided is a 1,4-dioxane-degrading bacteria culture method in which 1,4-dioxane-degrading bacteria are propagated using a medium containing diethylene glycol.
Opening claim text (preview).
What is claimed is: 1. A 1,4-dioxane-degrading bacteria culture method comprising culturing/incubating 1,4-dioxane-degrading bacteria with a medium containing diethylene glycol at a concentration of 1.0 wt % or more and 10.0 wt % or less, thereby multiplying the 1,4-dioxane-degrading bacteria, wherein the 1,4-dioxane-degrading bacteria are at least one of Mycobacterium sp. D11 (Accession Number: NITE BP-01926) or Pseudonocardia sp. D17 (Accession Number: NITE BP-01927). 2. The culture method according to claim 1 , wherein the medium is a liquid medium. 3. The culture method according to claim 2 , wherein the 1,4-dioxane-degrading bacteria are cultured/incubated with the liquid medium, obtaining culture solution, wherein the same amount of culture solution as the supply amount of liquid medium is removed while the liquid medium is supplied. 4. The culture method according to claim 2 , wherein the medium contains at least one component selected from the group consisting of corn steep liquor, casamino acid, yeast extract, and peptone. 5. The culture method according to claim 1 , wherein the medium contains at least one component selected from the group consisting of corn steep liquor, casamino acid, yeast extract, and peptone. 6. The culture method according to claim 5 , wherein the 1,4-dioxane-degrading bacteria are cultured/incubated with the liquid medium, obtaining culture solution, wherein the same amount of culture solution as the supply amount of liquid medium is removed while the liquid medium is supplied.
Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) · CPC title
characterised by the microorganisms used · CPC title
Biological treatment of water, waste water, or sewage {(C02F1/006 takes precedence)} · CPC title
microbiologically, {biologically} or by using enzymes · CPC title
Bacteria; Culture media therefor · CPC title
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