Methods and apparatus for shifted-wavelength photosynthetic energy harvesting and biomass production

Patent metadata

Field	Value
Publication number	US-10364410-B2
Application number	US-201715483572-A
Country	US
Kind code	B2
Filing date	Apr 10, 2017
Priority date	Apr 13, 2016
Publication date	Jul 30, 2019
Grant date	Jul 30, 2019

Abstract

One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.

First claim

What is claimed is:

1. A method comprising applying stimuli to a colony of organisms, such that:
(a) the stimuli include illuminating the colony with visible light and with light that has a wavelength that is outside of the range of wavelengths of visible light; and
(b) the stimuli, taken together, tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from light that has a wavelength that is outside of the range of wavelengths of visible light, wherein the illuminating comprises:
(i) illuminating the entire colony of organisms with green light;
(ii) illuminating a first portion of the colony, but not a second portion of the colony, with ultraviolet light; and
(iii) illuminating the second portion of the colony, but not the first portion of the colony, with infrared light.

2. The method of claim 1 , wherein the infrared light is pulsed.

3. The method of claim 1 , wherein the ultraviolet light is pulsed.

4. The method of claim 1 , wherein the organisms comprise algae.

5. The method of claim 1 , wherein the organisms comprise bacteria.

6. The method of claim 1 , wherein the stimuli, taken together, cause selective breeding or mutation of the organisms, such that, after the stimuli are applied to multiple generations of the organisms, a majority of individual organisms in the colony are adapted to perform photosynthesis which involves absorbing energy from light that has a wavelength that is outside of the range of wavelengths of visible light.

7. The method of claim 1 , wherein the method is performed with an Apparatus comprising:
(a) one or more light sources;
(b) one or more chambers; and
(c) one or more computers; wherein
(i) the one or more chambers are configured to contain the colony of organisms, and
(ii) the one or more computers are programmed to control the one or more light sources, such that the one or more light sources apply at least some of the stimuli to the colony by
(A) emitting the green light that illuminates the entire colony,
(B) emitting the ultraviolet light that illuminates the first portion of the colony but does not illuminate the second portion of the colony, and
(C) emitting the infrared light that illuminates the second portion of the colony but does not illuminate the first portion of the colony.

8. The method of claim 7 , wherein the one or more chambers each comprise one or more microfluidic channels.

9. The method of claim 7 , wherein the one or more chambers each comprise one or more petri dishes.

10. The method of claim 7 , wherein the one or more light sources each comprise one or more light emitting diodes.

11. The method of claim 7 , wherein the one or more light sources each comprise one or more lasers.

12. The method of claim 7 , wherein the one or more computers are programmed to control the one or more light sources, such that the stimuli are applied to multiple generations of the organisms.

13. The method of claim 12 , wherein the set of stimuli, taken together, cause selective breeding or mutation of the organisms such that, after the stimuli are applied to multiple generations of the organisms, a majority of individual organisms in the colony are adapted to perform photosynthesis that involves absorbing energy from light that has a wavelength that is outside of the range of wavelengths of visible light.

Assignees

• Massachusetts Inst Technology

Inventors

• Mershin Andreas

Classifications

• C12M35/02

  Electrical or electromagnetic means, e.g. for electroporation or for cell fusion · CPC title

• C12M31/02

  located outside the reactor · CPC title

• C12M23/10 Primary

  Petri dish (crystallising dishes B01L3/06) · CPC title

• C12M41/06

  of illumination · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title