Horizontal electrophoresis separation device without seal and method of extracting gel without opening cassette

Patent metadata

Field	Value
Publication number	US-10359397-B2
Application number	US-201514968400-A
Country	US
Kind code	B2
Filing date	Dec 14, 2015
Priority date	Dec 16, 2014
Publication date	Jul 23, 2019
Grant date	Jul 23, 2019

Abstract

Horizontal gel electrophoresis devices and methods of extracting a gel from a gel housing are provided. The devices allow for extraction of gels without having to open the housing comprising the gel. The devices include gel cassettes that simplify the automation of gel electrophoresis and electroblotting of proteins. Also provided are devices that allow for horizontal gel electrophoresis without the need to from a liquid seal between the buffer reservoir and the gel housing. Also described are methods for extracting a gel from a housing without opening the housing.

First claim

What is claimed is:

1. A device for horizontal electrophoresis, the device comprising:
a first and second chamber for holding buffer, the first and second chamber separated from each other and each comprising a floor, wherein the floor of the first and second chamber each comprises one or more opening(s) providing fluid communication between the first and second chambers and one or more separation channel(s) comprising an electrophoresis gel;
the separation channel(s) residing below the floor of the first and second chambers, wherein the separation channel(s) has a first and second end, the first end of said separation channel(s) abutting an extrusion member capable of sliding to extrude the electrophoresis gel in the separation channel out of the second end of the separation channel(s);
a cathode disposed within the first chamber and an anode disposed within the second chamber; and
a track aligned with each separation channel to receive the electrophoresis gel extruded by the extrusion member from the separation channel.

2. The device of claim 1 , wherein the openings in the floor of at least one of the first and second chambers form sample wells that protrude above a surface of the floor such that a volume of sample can be contained in each well.

3. The device of claim 1 , further comprising a first transfer electrode positioned above the track and a second transfer electrode positioned below the track, wherein the track is part of a solid support comprising pores and a medium for conducting ions.

4. The device of claim 1 , wherein the floor of the first or second chamber, and/or a bottom and/or a top of the separation channel comprises a transparent material.

5. The device of claim 1 , wherein the device comprises a plurality of separation channels.

6. The device of claim 1 , wherein the first and second chamber are separated from each other by a dividing wall, or the first and second chamber are horizontally separated from each other such that at least a portion of the separation channel is visible between the first and second chambers.

7. The device of claim 1 , wherein the first and/or second chamber further comprises an inlet and an outlet configured to circulate buffer by way of a pump that flows buffer from the inlet to the outlet.

8. A method for analyzing an analyte following gel electrophoresis, the method comprising:
extruding an electrophoresis gel comprising analytes separated by size or charge from the separation channel of the device of claim 1 onto a track aligned with the separation channel;
contacting the extruded electrophoresis gel with an electric field between first and second transfer electrodes such that the analytes are electrophoretically transferred to a solid support; and
analyzing the analyte.

9. The method of claim 8 , wherein the electrophoresis gel is extruded by contacting the electrophoresis gel with an extrusion member with sufficient force to push the electrophoresis gel out of the separation channel onto the track.

10. A system for aligning images of an electrophoresis gel, comprising:
the device of claim 1 wherein the electrophoresis gel comprises a dye band;
a detector capable of detecting the dye band; and
a computer configured with executable instructions for aligning a first image of the electrophoresis gel and a second image of the electrophoresis gel,
wherein the first image is obtained before the electrophoresis gel is extruded from the separation channel(s), and the second image is obtained after the electrophoresis gel is extruded from the separation channel(s), and the images are aligned based on a position of the dye band before the electrophoresis gel is extruded.

11. The device of claim 1 , wherein the separation channel(s) are electrically insulated from each other, and are open at each end such that the electrophoresis gel is exposed at both ends of the separation channel.

Assignees

• Bio Rad Laboratories

Inventors

• Uri Yochanan
• Issman Uri
• Kochetkov Igor
• Barak Itay
• Aharon Shai
• Nimri Shai

Classifications

• G01N27/44721

  by optical means · CPC title

• G01N27/44782

  of a plurality of samples · CPC title

• G01N27/44743

  Introducing samples · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title

• G01N27/44739 Primary

  Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots · CPC title