Pesticidal genes and methods of use

Patent metadata

Field	Value
Publication number	US-10358654-B2
Application number	US-201615530267-A
Country	US
Kind code	B2
Filing date	Dec 16, 2016
Priority date	Dec 22, 2015
Publication date	Jul 23, 2019
Grant date	Jul 23, 2019

Abstract

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Polynucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

First claim

That which is claimed is:

1. A recombinant polypeptide having pesticidal activity, comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence-set forth in SEQ ID NO:58, wherein said polypeptide further comprises a heterologous amino acid sequence chemically linked to said polypeptide.

2. A composition comprising the polypeptide of claim 1 .

3. A nucleic acid molecule encoding a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:58, wherein said polypeptide has pesticidal activity, and wherein said nucleic acid molecule is operably linked to a heterologous promoter.

4. The nucleic acid molecule of claim 3 , wherein said nucleic acid molecule is a synthetic sequence designed for expression in a plant.

5. The nucleic acid molecule of claim 3 , wherein said heterologous promoter is capable of directing expression in a plant cell.

6. The nucleic acid molecule of claim 3 , wherein said heterologous promoter is capable of directing expression in a bacterial cell.

7. A host cell comprising the nucleic acid molecule of claim 3 .

8. The host cell of claim 7 , wherein said host cell is a bacterial host cell.

9. A vector comprising a DNA construct comprising a heterologous promoter that drives expression in a plant cell operably linked to a nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO: 58, wherein said polypeptide has pesticidal activity.

10. The vector of claim 9 , wherein said nucleotide sequence is a synthetic DNA sequence designed for expression in a plant.

11. A host cell comprising the vector of claim 9 .

12. A composition comprising the host cell of claim 11 .

13. A method for controlling a pest population comprising contacting said pest population with a pesticidal-effective amount of the composition of claim 2 .

14. A method for producing a polypeptide with pesticidal activity comprising culturing the host cell of claim 7 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

15. A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO: 58, wherein said polypeptide has pesticidal activity.

16. A transgenic seed of the plant of claim 15 , wherein the transgenic seed comprises in its genome the DNA construct.

17. A method for protecting a plant from an insect pest, comprising expressing in a plant or cell thereof a nucleotide sequence that encodes a pesticidal polypeptide, wherein said pesticidal polypeptide has at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO: 58.

18. The method of claim 17 , wherein said pesticidal polypeptide has pesticidal activity against at least one of a lepidopteran pest, a coleopteran pest, or a hemipteran pest.

19. A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence comprises:
(a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 58; or
(b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid set forth in SEQ ID NO:58.

20. The recombinant polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 58.

21. The recombinant polypeptide of claim 1 , wherein the polypeptide comprises the amino acid sequence set forth in SEQ ID NO:58.

22. The nucleic acid molecule of claim 3 , wherein the nucleic acid molecule encodes a polypeptide comprising at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:58, wherein said polypeptide has pesticidal activity.

23. The nucleic acid molecule of claim 3 , wherein the nucleic acid molecule encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 58.

24. The vector of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:58.

25. The DNA construct of claim 9 , wherein the nucleic acid molecule comprises a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:58.

26. The plant of claim 15 , wherein the DNA construct comprises a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 58, wherein said polypeptide has pesticidal activity.

27. The plant of claim 15 , wherein the DNA construct comprises a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:58.

28. The method of claim 17 , wherein said nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 58.

29. The method of claim 17 , wherein said nucleotide sequence encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:58.

Assignees

• Agbiome Inc

Inventors

• Parks Jessica
• Roberts Kira Bulazel
• Thayer Rebecca E

Classifications

• C07K14/195 Primary

  from bacteria · CPC title

• A01N37/46

  N-acyl derivatives · CPC title

• C07K14/32

  from Bacillus (G) · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title