Antibody neutralizing human respiratory syncytial virus

Patent metadata

Field	Value
Publication number	US-10358480-B2
Application number	US-201815928438-A
Country	US
Kind code	B2
Filing date	Mar 22, 2018
Priority date	Oct 29, 2015
Publication date	Jul 23, 2019
Grant date	Jul 23, 2019

Abstract

The present invention relates to monoclonal antibodies which have high anti-RSV neutralizing titers. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. The invention yet further provides for diagnostic, prophylactic and therapeutic methods employing the antibodies and nucleic acids of the invention, particularly as a passive immunotherapy agent in infants and the elderly.

First claim

The invention claimed is:

1. An isolated nucleic acid molecule encoding an antibody heavy chain variable region comprising the amino acid sequence of SEQ ID NO:7, an antibody light chain variable region comprising the amino acid sequence of SEQ ID NO:8, or encoding both an antibody heavy chain variable region comprising the amino acid sequence of SEQ ID NO:7 and an antibody light chain variable region comprising the amino acid sequence of SEQ ID NO:8.

2. The isolated nucleic acid molecule of claim 1 , wherein the isolated nucleic acid molecule encodes an antibody heavy chain comprising the amino acid sequence of SEQ ID NO:23, an antibody light chain comprising the amino acid sequence of SEQ ID NO:25, or encoding both an antibody heavy chain comprising the amino acid sequence of SEQ ID NO:23 and an antibody light chain comprising the amino acid sequence of SEQ ID NO:25.

3. The isolated nucleic acid molecule of claim 2 , wherein the nucleic acid molecule encoding the antibody heavy chain has the nucleic acid sequence set forth in SEQ ID NO:24 and the nucleic acid molecule encoding the antibody light chain has the nucleic acid sequence set forth in SEQ ID NO:26.

4. An isolated nucleic acid molecule comprising a nucleic acid sequence set forth in SEQ ID NO:17.

5. An expression vector comprising the nucleic acid molecule of claim 1 , 2 , 3 or 4 .

6. A host cell comprising the expression vector of claim 5 .

7. The host cell of claim 6 , wherein the host cell is a Pichia cell or a Chinese hamster ovary cell.

Assignees

• Merck Sharp & Dohme

Inventors

• Vora Kalpit A
• Cox Kara S
• Tang Aimin
• Chen Zhifeng
• Distefano Daniel
• Zhang Lan
• Su Hua-Poo

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P37/06

  Immunosuppressants, e.g. drugs for graft rejection · CPC title

• A61P31/22

  for herpes viruses · CPC title

• A61P31/20

  for DNA viruses · CPC title

• A61P31/16

  for influenza or rhinoviruses · CPC title