Engineered microbe-targeting molecules and uses thereof
US-2017362307-A1 · Dec 21, 2017 · US
Modified lectin derived from Wisteria floribunda
US10358466B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10358466-B2 |
| Application number | US-201715706144-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 15, 2017 |
| Priority date | Dec 21, 2012 |
| Publication date | Jul 23, 2019 |
| Grant date | Jul 23, 2019 |
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Abstract
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A Wisteria floribunda monomeric lectin polypeptide is provided. The Wisteria floribunda monomeric lectin polypeptide includes any one of the amino acid sequences selected from the group consisting of: (1) the amino acid sequence represented by SEQ ID NO: 2; (2) the amino acid sequence defined in (1) above, except that one to 20 amino acids at positions other than Cys272 position is/are deleted, substituted, inserted, or added; and (3) the amino acid sequence defined in (1) or (2) above, further having an N-terminus deletion of one to 30 amino acids, in which Cys272 is alkylated, and the polypeptide is capable of specifically binding to a GalNAc terminal sugar chain.
First claim
Opening claim text (preview).
What is claimed is: 1. A Wisteria floribunda monomeric lectin polypeptide comprising any one of the amino acid sequences selected from the group consisting of: (1) the amino acid sequence represented by SEQ ID NO: 2; (2) the amino acid sequence defined in (1) above, except that one to 20 amino acids at positions other than Cys272 position is/are deleted, substituted, inserted, or added; and (3) the amino acid sequence defined in (1) or (2) above, further having an N-terminus deletion of one to 30 amino acids, wherein Cys272 of each of the amino acid sequences defined in (1)-(3) above is alkylated, and wherein the polypeptide is capable of specifically binding to a GalNAc terminal sugar chain. 2. A reagent composition for detecting a GalNAc terminal sugar chain marker, wherein the reagent composition comprises the polypeptide of claim 1 . 3. A method for producing a Wisteria floribunda monomeric lectin polypeptide with alkylated Cys272 residue capable of specifically binding to a GalNAc terminal sugar-chain, the method comprising: (a) reacting a Wisteria floribunda dimeric lectin polypeptide with a reducing agent, and (b) reacting the reduced Wisteria floribunda dimeric lectin polypeptide with an alkylating agent to produce the Wisteria floribunda monomeric lectin polypeptide with the alkylated Cys272 residue, wherein the Wisteria floribunda dimeric lectin polypeptide utilized in the step (a) comprises any one of the amino acid sequences selected from the group consisting of: (1) the amino acid sequence represented by SEQ ID NO: 2; (2) the amino acid sequence defined in (1) above, except that one to 20 amino acids at positions other than Cys272 position is/are deleted, substituted, inserted, or added; and (3) the amino acid sequence defined in (1) or (2) above, further having an N-terminus deletion of one to 30 amino acids.
Assignees
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Classifications
- C07K14/42Primary
Lectins, e.g. concanavalin, phytohaemagglutinin · CPC title
for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title
Assays, e.g. immunoassays or enzyme assays, involving carbohydrates · CPC title
Lectins, e.g. concanavalin, phytohaemagglutinin · CPC title
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- What does patent US10358466B2 cover?
- A Wisteria floribunda monomeric lectin polypeptide is provided. The Wisteria floribunda monomeric lectin polypeptide includes any one of the amino acid sequences selected from the group consisting of: (1) the amino acid sequence represented by SEQ ID NO: 2; (2) the amino acid sequence defined in (1) above, except that one to 20 amino acids at positions other than Cys272 position is/are deleted,…
- Who is the assignee on this patent?
- Aist, National Institute Of Advanced Industrial Science & Tech
- What technology area does this patent fall under?
- Primary CPC classification C07K14/42. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 23 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).