Preparation and use of a composition for prevention and mitigation of the effects of radiation

Patent metadata

Field	Value
Publication number	US-10357474-B2
Application number	US-201815996101-A
Country	US
Kind code	B2
Filing date	Jun 1, 2018
Priority date	Sep 13, 2013
Publication date	Jul 23, 2019
Grant date	Jul 23, 2019

Abstract

Plant extracts, compositions, pharmaceutical compositions and methods of making and using the same are provided herein. The compositions comprise γ-tocotrienol (GT3) and δ-tocotrienol (DT3) in ratios wherein the DT3 is predominate. The compositions are useful for radioprotection and radiomitigation in subjects in need thereof.

First claim

We claim:

1. A method of treating a subject in need of a radioprotectant or for treatment of radiation exposure, the method comprising administering an effective amount of a tocotrienol composition comprising δ-tocotrienol and γ-tocotrienol in a ratio of between 2:1 and 10:1.

2. The method of claim 1 , wherein the tocotrienol composition comprises δ-tocotrienol and γ-tocotrienol in a ratio of between 5:1 and 9:1.

3. The method of claim 1 , wherein the tocotrienol composition comprises an annatto seed extract.

4. The method of claim 3 , wherein the annatto seed extract is prepared from a method comprising:
extracting oil from annatto seeds to produce an annatto seed oil, the annatto seed oil comprising δ-tocotrienol and γ-tocotrienol, and
enriching δ-tocotrienol or γ-tocotrienol from the annatto seed oil.

5. The method of claim 4 , wherein the method comprises enriching δ-tocotrienol and γ-tocotrienol from the annatto seed oil.

6. The method of claim 1 , wherein the tocotrienol composition comprises a total tocotrienol content of between 15% and 95%.

7. The method of claim 6 , wherein the total tocotrienol content is at least 50%.

8. The method of claim 1 , wherein the tocotrienol composition comprises a total tocotrienol content of at least 100 mg/mL.

9. The method of claim 8 , wherein the total tocotrienol content is at least 500 mg/mL.

10. The method of claim 1 , wherein the tocotrienol composition comprises a tocopherol content of less than 5%.

11. The method of claim 10 , wherein the tocopherol content is less than 1%.

12. The method of claim 1 , wherein the tocotrienol composition comprises a total tocotrienol content of between 15% and 95% and a tocopherol content of less than 5%.

13. The method of claim 2 , wherein the tocotrienol composition comprises an annatto seed extract having δ-tocotrienol and γ-tocotrienol in a ratio of between 5:1 and 9:1.

14. The method of claim 1 , wherein the tocotrienol composition is a pharmaceutical composition further comprising a pharmaceutically acceptable carrier.

15. The method of claim 14 , wherein the pharmaceutical composition comprises a dosage form having a total tocotrienol content of least 1 g.

16. The method of claim 15 , wherein the pharmaceutical composition comprises an annatto seed extract.

17. The method of claim 16 , wherein the annatto seed extract is prepared from a method comprising:
extracting oil from annatto seeds to produce an annatto seed oil, the annatto seed oil comprising δ-tocotrienol and γ-tocotrienol, and
enriching δ-tocotrienol or γ-tocotrienol from the annatto seed oil.

Assignees

• Bioventures Llc
• Univ Tecnica Particular De Loja
• Us Veterans Affairs

Inventors

• Compadre Cesar M
• Breen Philip
• Aykin-Burns Nukhet
• Hauer-Jensen Martin
• Enriquez Raul G
• Kharade Sujay
• Malagon Omar
• Ordonez Yadira
• Ojeda Edgar
• Thakkar Shraddha
• Pineda E Nathalie
• Jones Darin

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P39/06

  Free radical scavengers or antioxidants · CPC title

• A61P39/00

  General protective or antinoxious agents · CPC title

• A61P37/02

  Immunomodulators · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title