Engineering cyclopropane fatty acid accumulation in plants

Patent metadata

Field	Value
Publication number	US-10351868-B2
Application number	US-201414914532-A
Country	US
Kind code	B2
Filing date	Aug 26, 2014
Priority date	Aug 28, 2013
Publication date	Jul 16, 2019
Grant date	Jul 16, 2019

Abstract

Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.

First claim

Whatis claimed is:

1. A transgenic plant comprising higher levels of oleic acid than a wild type plant wherein said transgenic plant is a fad2fae1 plant that expresses Escherichia coli cyclopropane synthase and Sterculia foetida diacylglycerol acyltransferase (DGAT) and accumulates elevated amounts of cyclopropane fatty acids, wherein the transgenic plant is an oilseed crop plant selected from the group consisting of soybean, safflower, sunflower, canola, palm, Brassica , Cranbe and Camelina.

2. The transgenic plant of claim 1 that accumulates cyclopropane fatty acids in its seed.

3. The seed of the transgenic plant of claim 1 wherein the cyclopropane fatty acids comprise between about five percent (5%) and about sixty percent (60%) of the total seed fatty acid.

4. The seed of the transgenic plant of claim 1 wherein the cyclopropane fatty acids comprise between about ten percent (10%) and about forty percent (40%) of the total seed fatty acid.

5. A method producing cyclopropane fatty acids in an oilseed crop plant, the method comprising transforming fad2fae1 plant having higher levels of oleic acid than a wild type plant with a construct comprising a nucleic acid sequence encoding an Escherichia coli cyclopropane synthase and a nucleic acid sequence encoding a Sterculia foetida diacylglycerol acyltransferase (DGAT), wherein the oilseed crop plant accumulates elevated amounts of cyclopropane fatty acids, wherein the transgenic plant is an oilseed crop plant selected from the group consisting of soybean, safflower, sunflower, canola, palm, Brassica , Cranbe and Camelina.

6. The method of claim 5 , wherein the transgenic plant accumulates cyclopropane fatty acids in its seed.

7. The method of claim 5 , wherein the cyclopropane fatty acids comprise between about five percent (5%) and about sixty percent (60%) of the total seed fatty acid.

8. The method of claim 5 wherein the cyclopropane fatty acids comprise between about ten percent (10%) and about forty percent (40%) of the total seed fatty acid.

Assignees

• Brookhaven Science Ass Llc

Inventors

• Shanklin John
• Yu Xiao-Hong
• Prakash Richa Rawat

Classifications

• C12N9/1007

  Methyltransferases (general) (2.1.1.) · CPC title

• C12P7/6463

  obtained from glyceride producing microorganisms, e.g. single cell oil · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12P7/6409

  Fatty acids · CPC title