Preparation of poly alpha-1,3-glucan ethers
US-2015353649-A1 · Dec 10, 2015 · US
US10351633B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10351633-B2 |
| Application number | US-201515313347-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 22, 2015 |
| Priority date | May 29, 2014 |
| Publication date | Jul 16, 2019 |
| Grant date | Jul 16, 2019 |
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An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.
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What is claimed is: 1. A method to produce an alpha-glucan, said method comprising: (a) combining at least sucrose and an isolated polypeptide comprising an amino acid sequence having at least 95% identity to SEQ ID NO:10 under suitable aqueous reaction conditions to catalyze synthesis of water-soluble alpha-glucan comprising 100% alpha-1,6 glycosidic linkages, and (b) optionally isolating the water-soluble alpha-glucan produced in step (a). 2. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 96% identity to SEQ ID NO:10. 3. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 97% identity to SEQ ID NO:10. 4. The method of claim 1 , wherein the method comprises step (b) of isolating the water-soluble alpha-glucan produced in step (a). 5. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 98% identity to SEQ ID NO:10. 6. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 99% identity to SEQ ID NO:10.
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1) · CPC title
Dextransucrase (2.4.1.5) · CPC title
Glycosyltransferases (2.4) · CPC title
produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title
Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00 · CPC title
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