Methods, arrangements and systems for obtaining information associated with an anatomical sample using optical microscopy

Patent metadata

Field	Value
Publication number	US-10351616-B2
Application number	US-201715690403-A
Country	US
Kind code	B2
Filing date	Aug 30, 2017
Priority date	Feb 8, 2006
Publication date	Jul 16, 2019
Grant date	Jul 16, 2019

Abstract

Arrangements and methods are provided for obtaining information associated with an anatomical sample. For example, at least one first electro-magnetic radiation can be provided to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample. At least one second electro-magnetic radiation can be produced based on the acoustic wave. At least one portion of at least one second electro-magnetic radiation can be provided so as to determine information associated with at least one portion of the anatomical sample. In addition, the information based on data associated with the second electro-magnetic radiation can be analyzed. The first electro-magnetic radiation may include at least one first magnitude and at least one first frequency. The second electro-magnetic radiation can include at least one second magnitude and at least one second frequency. The data may relate to a first difference between the first and second magnitudes and/or a second difference between the first and second frequencies. The second difference may be approximately between −100 GHz and 100 GHz, excluding zero.

First claim

What is claimed is:

1. An arrangement comprising:
at least one first arrangement configured to provide at least one first electro-magnetic radiation to an anatomical sample so as to generate at least one acoustic wave in the anatomical sample, wherein at least one second electro-magnetic radiation is produced based on the at least acoustic wave;
at least one second arrangement configured to receive at least one portion of the at least one second electro-magnetic radiation; and
at least one third arrangement configured to determine information associated with a biomechanical property of at least one portion of the anatomical sample based on a measurement of at least one of a magnitude or frequency of a spectral peak in a spectrum of the at least one second electro-magnetic radiation.

2. The arrangement according to claim 1 , wherein the anatomical sample is at least one of an organ, a tissue or cell.

3. The arrangement according to claim 1 , further comprising at least one fourth arrangement configured to image the at least one portion of the anatomical sample based on data associated with the at least one second electro-magnetic radiation.

4. The arrangement according to claim 3 , further comprising at least one fifth arrangement configured to translate the at least one electro-magnetic radiation across the anatomical sample.

5. The arrangement according to claim 4 , wherein the at least one fifth arrangement includes at least one lens, and wherein the at least one lens focuses the at least one first electro-magnetic radiation and collects the at least one second electro-magnetic radiation.

6. The arrangement according to claim 3 , wherein the at least one first electro-magnetic radiation includes at least one first magnitude and at least one first frequency, wherein the at least one second electro-magnetic radiation includes at least one second magnitude and at least one second frequency, and wherein the data relates to at least one of a first difference between the first and second magnitudes or a second difference between the first and second frequencies.

7. The arrangement according to claim 6 , wherein the second difference is between −100 GHz and 100 GHz, excluding zero.

8. The arrangement according to claim 1 , wherein the at least one second arrangement includes a spectral filter which facilitates a determination of the spectrum of the at least one second electro-magnetic radiation.

9. The arrangement according to claim 4 , wherein the at least one fifth arrangement is at least one of a catheter, an endoscope, a laparoscope, or an ophthalmic visualization device.

10. The arrangement according to claim 1 , wherein the information is associated with a biomechanical property of the anatomical sample.

11. The arrangement according to claim 1 , wherein the anatomical sample is a living subject.

12. The arrangement according to claim 1 , wherein the at least one first electromagnetic radiation has a center wavelength which is between 0.5-1.8 μm.

13. The arrangement according to claim 12 , wherein the at least one first electromagnetic radiation has a line width which is smaller than 100 MHz.

14. The arrangement according to claim 12 , wherein the at least one first electromagnetic radiation has a form of a single continuous wave.

15. The arrangement according to claim 1 , wherein the at least one first electro-magnetic radiation includes at least two first electro-magnetic radiations, wherein a first one of the at least two first electro-magnetic radiations has a first frequency, and a second one of the at least two first electro-magnetic radiations has a second frequency, and wherein a difference between the first and second frequencies is between zero and 100 GHz.

16. An arrangement comprising:
at least one first arrangement configured to provide at least one first electro-magnetic radiation to an anatomical sample, wherein at least one second electro-magnetic radiation is provided from the anatomical sample;
at least one second arrangement configured to receive at least one portion of the at least one second electro-magnetic radiation; and
at least one third arrangement configured to determine information associated with a biomechanical property of at least one portion of the anatomical sample based on a measurement of at least one of a magnitude or frequency of a spectral peak in a spectrum of the at least one second electro-magnetic radiation,
wherein the at least one first electro-magnetic radiation includes at least one first magnitude and at least one first frequency, wherein the at least one second electro-magnetic radiation includes at least one second magnitude and at least one second frequency, and wherein the data relates to at least one of a first difference between the first and second magnitudes or a second difference between the first and second frequencies, and wherein the second difference is between −100 GHz and 100 GHz, excluding zero.

17. The arrangement according to claim 16 , further comprising at least one fourth arrangement configured to image the at least one portion of the anatomical sample based on data associated with the at least one second electro-magnetic radiation.

18. A method for obtaining information associated with an anatomical sample, comprising:
providing at least one first electro-magnetic radiation to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample, wherein at least one second electro-magnetic radiation is produced based on the at least acoustic wave;
receiving at least one portion of at least one second electro-magnetic radiation; and
determining information associated with a biomechanical property of at least one portion of the anatomical sample based on a measurement of at least one of a magnitude or frequency of a spectral peak in a spectrum of the at least one second electro-magnetic radiation.

19. The arrangement according to claim 1 , wherein the at least one first arrangement forms a pump oscillator including a single laser configured to deliver the at least one first electro-magnetic radiation to the anatomical sample.

20. The arrangement according to claim 19 , further comprising a local oscillator including a laser configured to provide a reference light to be processed by the at least one second arrangement configured to receive the at least one second electro-magnetic radiation.

Assignees

• Massachusetts Gen Hospital

Inventors

• Yun Seok-Hyun

Classifications

• G01N21/1717

  with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance · CPC title

• C07K2319/70

  containing domain for protein-protein interaction · CPC title

• G01N21/636

  using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties; (non-linear optics per se G02F1/35) · CPC title

• A61B5/0097

  by applying acoustic waves and detecting light, i.e. acousto-optic measurements · CPC title

• G01N2800/7028

  Cancer · CPC title