Peptide and complex of same, scaffold for tissue repair and surface treatment method for same, and surface treatment solution or set of treatment solutions

Patent metadata

Field	Value
Publication number	US-10351600-B2
Application number	US-201715663043-A
Country	US
Kind code	B2
Filing date	Jul 28, 2017
Priority date	Apr 26, 2013
Publication date	Jul 16, 2019
Grant date	Jul 16, 2019

Abstract

A peptide that enables surface treatment of a scaffold for tissue repair that makes it possible to accelerate the repair of living tissue without using a material that negatively affects the repair of living tissue, a complex containing this peptide, a scaffold for tissue repair surface treated using this peptide or this complex, a surface treatment method for a scaffold for tissue repair using this peptide or this complex, and a treatment solution or set of treatment solutions to be used in this surface treatment method. Surface treatment of a scaffold for tissue repair is conducted by combining glycosaminoglycan and a peptide containing adhesive sites and basic sites each comprising predetermined amino acid residues.

First claim

We claim:

1. A complex of peptide and glycosaminoglycan,
wherein the peptide comprises, in the amino acid sequence thereof, an adhesive site consisting of residues of amino acids including a 2,3-dihydroxyphenyl group or 3,4-dihydroxyphenyl group, and a basic site including three or more consecutive basic amino acid residues;
wherein the adhesive site and the basic site bind via a spacer site, and
wherein the glycosaminoglycan is binding at the basic site.

2. The complex according to claim 1 , wherein a sequence of nine resides from the C terminus of the peptide is an amino acid sequence represented by SEQ ID NO 1.

3. A complex of the complex according to claim 1 and a cell growth factor,
wherein the cell growth factor is binding with the glycosaminoglycan.

4. A scaffold for tissue repair, wherein the complex according to claim 1 is binding to the surface thereof at the adhesive site.

5. A scaffold for tissue repair, wherein a complex of the complex according to claim 1 and a cell growth factor are binding to a surface of the scaffold at the adhesive site.

6. A treatment solution for surface treatment of a scaffold for tissue repair, comprising the complex according to claim 1 .

7. A set of treatment solutions for surface treatment of a scaffold for tissue repair, comprising the treatment solution according to claim 6 and a treatment solution containing cell growth factor.

8. A treatment solution for surface treatment of a scaffold for tissue repair, comprising a complex of the complex according to claim 1 and a cell growth factor.

Assignees

• Nat Cerebral & Cardiovascular Ct
• Jms Co Ltd

Inventors

• Yamaoka Tetsuji
• Kakinoki Sachiro
• Baba Shunsuke
• Hashimoto Yoshiya

Classifications

• C07K14/50

  Fibroblast growth factor [FGF] · CPC title

• A61L2300/414

  Growth factors · CPC title

• A61L27/12

  Phosphorus-containing materials, e.g. apatite · CPC title

• C07K9/00

  Peptides having up to 20 amino acids, containing saccharide radicals and having a fully defined sequence; Derivatives thereof · CPC title

• A61L2300/25

  Peptides having up to 20 amino acids in a defined sequence · CPC title