Method and apparatus for manipulating particles

Patent metadata

Field	Value
Publication number	US-10350613-B2
Application number	US-201414911839-A
Country	US
Kind code	B2
Filing date	May 8, 2014
Priority date	Aug 14, 2013
Publication date	Jul 16, 2019
Grant date	Jul 16, 2019

Abstract

A method and apparatus for manipulating polarizable dielectric particles. The method includes positioning a liquid containing the particles above a surface of a piezoelectric material (2). The method also includes inducing a shear-horizontal surface acoustic wave in the piezoelectric material (2), thereby to form a time-varying non-uniform evanescent electric field extending into the liquid. The method further includes using the time-varying non-uniform evanescent electric field to apply a force to at least some of the particles (50, 52) by dielectrophoresis.

First claim

The invention claimed is:

1. A method of manipulating polarizable dielectric particles, the method comprising:
positioning a liquid containing polarizable dielectric particles above a surface of a piezoelectric material, wherein a conductivity of the liquid is in the range of 0.001 to 2.0 S/m;
inducing a shear-horizontal surface acoustic wave in the piezoelectric material, thereby to form a time-varying non-uniform evanescent electric field extending into the liquid; and
using the time-varying non-uniform evanescent electric field to apply a force to at least some of the polarizable dielectric particles to manipulate the polarizable dielectric particles by dielectrophoresis.

2. The method of claim 1 , wherein the shear-horizontal surface acoustic wave is a composite wave comprising two components travelling in opposite directions in the piezoelectric material.

3. The method of claim 2 , wherein the shear-horizontal surface acoustic wave is a standing wave.

4. The method of claim 3 , wherein the liquid contains a plurality of types of polarizable dielectric particles, each type of polarizable dielectric particle having respective polarization properties, the method comprising sorting a plurality of polarizable dielectric particles of a first type from a plurality of polarizable dielectric particles of a second type by allowing the polarizable dielectric particles contained in the liquid to move toward regions of higher or lower electric field gradient according to whether they experience positive dielectrophoresis or negative dielectrophoresis.

5. The method of claim 4 , further comprising separating the plurality of polarizable dielectric particles of the first type from the plurality of polarizable dielectric particles of the second type by directing them along respective fluid channels after they have been sorted by dielectrophoresis in a region above the surface of the piezoelectric material.

6. The method of claim 2 , further comprising applying a force to the polarizable dielectric particles in the liquid by varying a frequency and/or phase of at least one of the two components of the composite shear-horizontal surface acoustic wave to reposition one or more nodes or antinodes of the time-varying evanescent electric field above the surface of the piezoelectric material.

7. The method of claim 1 , further comprising:
causing the liquid containing the polarizable dielectric particles to flow in a first direction above the surface of the piezoelectric material; and
sorting the polarizable dielectric particles contained in the liquid by applying a dielectrophoretic force to the polarizable dielectric particles in a second direction different from the first direction.

8. The method of claim 7 , comprising sorting the polarizable dielectric particles in the liquid according to an amount by which they are deflected as the liquid containing them traverses a region of the piezoelectric material.

9. The method of claim 1 , wherein the polarizable dielectric particles in the liquid comprise biological cells.

10. The method of claim 1 , further comprising selecting a particular conductivity of the liquid according to the Clausius-Mossotti factor of polarizable dielectric particles to be manipulated, for applying a force to at least some of the polarizable dielectric particles in the liquid either by positive or negative dielectrophoresis in the time-varying non-uniform evanescent electric field.

11. A particle manipulation apparatus for manipulating polarizable dielectric particles contained in a liquid, the apparatus comprising:
a substrate comprising a piezoelectric material that supports generation of shear-horizontal surface acoustic waves;
a liquid-receiving region located above a surface of the substrate;
a liquid contained in the liquid-receiving region, wherein the liquid contains polarizable dielectric particles and has a conductivity in the range of 0.001 to 2.0 S/m; and
a first transducer configured to induce a shear-horizontal surface acoustic wave in the piezoelectric material beneath the liquid-receiving region, thereby to form a time-varying non-uniform evanescent electric field extending into the liquid-receiving region for applying a force to at least some of the polarizable dielectric particles by dielectrophoresis.

12. The particle manipulation apparatus of claim 11 , wherein the liquid-receiving region comprises a channel through which the liquid containing the polarizable dielectric particles can flow.

13. The particle manipulation apparatus of claim 11 , wherein the liquid-receiving region is furcated at one end to define a plurality of branches, each branch for receiving polarizable dielectric particles manipulated by dielectrophoresis within the liquid-receiving region.

14. The particle manipulation apparatus of claim 11 , further comprising a second transducer configured to cooperate with the first transducer to induce a composite shear-horizontal surface acoustic wave comprising two components travelling in opposite directions in the piezoelectric material.

15. The particle manipulation apparatus of claim 14 , wherein the composite wave is a standing wave.

16. The particle manipulation apparatus of claim 14 further comprising circuitry for varying a frequency and/or phase of a signal applied to one or each of the first and second transducers to vary a frequency and/or phase of at least one of the two components of the composite shear-horizontal surface acoustic wave to reposition one or more nodes or antinodes of the time-varying evanescent electric field above the surface of the piezoelectric material.

17. The particle manipulation apparatus of claim 11 further comprising one or more reflectors positioned behind the first transducer to reflect a part of the surface acoustic wave induced by the first transducer back toward the liquid-receiving region.

18. The particle manipulation apparatus of claim 11 further comprising a waveguide layer located between the piezoelectric material of the substrate and the liquid-receiving region.

19. The particle manipulation apparatus of claim 11 further comprising one or more sensors positioned to sense a property of the polarizable dielectric particles in the liquid in the liquid-receiving region.

20. The particle manipulation apparatus of claim 11 , wherein the piezoelectric material comprises lithium tantalate, quartz, langasite, or lithium niobate.

Assignees

• Univ Leeds Innovations Ltd

Inventors

• Walti Christoph
• Smith Alban Josiah
• O'Rorke Richard
• Davies Alexander Giles
• Wood Christopher David

Classifications

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L2400/0439

  ultrasonic vibrations, vibrating piezo elements · CPC title

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• B03C5/022

  Non-uniform field separators · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title