Graphene-based ink compositions for three-dimensional printing applications

Patent metadata

Field	Value
Publication number	US-10350329-B2
Application number	US-201515500339-A
Country	US
Kind code	B2
Filing date	Oct 15, 2015
Priority date	Oct 15, 2014
Publication date	Jul 16, 2019
Grant date	Jul 16, 2019

Abstract

Electrically conducting, biocompatible, biodegradable tissue growth comprising graphene flakes in a polymeric matrix are provided.

First claim

What is claimed is:

1. An implantable tissue growth structure, wherein the structure is a three-dimensional, biocompatible, biodegradable, electrically conductive structure comprising a network of fibers, the fibers comprising a composite comprising graphene flakes in a polymeric matrix, wherein the composite has a graphene flake content of at least 40 vol. %.

2. The structure of claim 1 , wherein the fibers are arranged in an ordered, three-dimensional grid.

3. The structure of claim 1 , wherein the graphene flakes along the surfaces of the fibers are preferentially aligned along the long axes of the fibers.

4. The structure of claim 3 , wherein the fibers have higher electrical conductivities at their surfaces than in their bulk.

5. The structure of claim 1 , wherein the structure is seeded with stem cells.

6. The scaffold of claim 1 , wherein the polymeric matrix comprises polylactic acid, glycolic acid, copolymers of polylactic acid and glycolic acid, polycaprolactone, or a mixture thereof.

7. The structure of claim 1 , wherein the network of fibers comprises fibers having diameters of 93.5 μm or greater.

8. The structure of claim 7 , wherein the network of fibers comprises fibers having diameters in the range from 100 μm to 1000 μm.

9. The structure of claim 1 , wherein at least some of the fibers are fused with adjacent, non-parallel fibers.

10. A method for generating electrogenic cells or tissues, the method comprising:
contacting a three-dimensional, biocompatible, biodegradable, electrically conductive structure with electrogenic cells or electrogenic tissue, wherein the structure comprises a network of fibers, the fibers comprising a composite of graphene flakes in a polymeric matrix, and further wherein the composite has a graphene flake content of at least 40 vol. %, wherein electrogenic cell generation takes place on the scaffold.

11. The method of claim 10 further comprising contacting the structure with stem cells, wherein the electrogenic cell generation takes place by allowing the stem cells to differentiate into electrogenic cells.

12. The method of claim 11 , wherein the stem cells are human mesenchymal stem cells.

13. The method of claim 11 , wherein contacting the structure with stem cells comprises seeding the structure with stem cells, and further wherein allowing the stem cells to differentiate into electrogenic cells comprises culturing the cell-seeded structure in a culture medium.

14. The method of claim 13 , wherein the culture medium is free of neuronal differentiating biochemical factors.

15. The method of claim 11 , wherein contacting the structure with electrogenic cells or electrogenic tissue comprises implanting the structure into electrogenic tissue in a living subject.

16. The method of claim 15 , further comprising seeding the structure with stem cells prior to implanting it into the electrogenic tissue in the living subject, wherein signals from the electrogenic tissue induce the differentiation of the stem cells into electrogenic cells.

17. The method of claim 16 , wherein the electrogenic cells are neurogenic cells.

18. The method of claim 17 , wherein the structure is characterized in that it is able to induce neurogenic differentiation without the need for neuronal differentiating biochemical factors.

19. The method of claim 17 , wherein the composite has a graphene flake content of at least 60 vol. %.

20. The method of claim 17 , wherein the stem cells are human mesenchymal stem cells.

21. The method of claim 15 , wherein the structure is implanted such that it connects a nerve end or nerve bundle to another nerve end or nerve bundle, or to electrogenic tissue in the living subject, wherein the structure acts as a nerve guide by directing nerve generation along its length.

22. The method of claim 15 , wherein the scaffold is implanted such that it connects a first segment of muscle tissue to a second segment of muscle tissue in the living subject, wherein the scaffold acts as a tissue growth guide by directing muscle tissue generation along its length.

23. The method of claim 22 , wherein the muscle tissue is cardiac tissue.

Assignees

• Univ Northwestern

Inventors

• Shah Ramille N
• Jakus Adam E
• Hersam Mark C
• Secor Ethan B

Classifications

• A61L27/08

  Carbon {; Graphite} · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• C09D11/10

  based on artificial resins · CPC title

• C09D11/037

  characterised by the pigment · CPC title

• A61L27/38

  containing added animal cells (organs or tissue containing native cells A61L27/36) · CPC title