Compositions, devices, systems, and methods for using a nanopore

Patent metadata

Field	Value
Publication number	US-10344327-B2
Application number	US-201615087734-A
Country	US
Kind code	B2
Filing date	Mar 31, 2016
Priority date	Apr 4, 2007
Publication date	Jul 9, 2019
Grant date	Jul 9, 2019

Abstract

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

First claim

What is claimed is:

1. A method for analyzing a plurality of molecules, comprising:
(a) forming a plurality of individually addressable nanopore devices on a chip, each individually addressable nanopore device of said plurality of individually addressable nanopore devices containing at least one nanopore formed in a membrane disposed adjacent to an electrode, each individually addressable nanopore device being addressable using row and column addressing;
(b) directing a plurality of molecules to said individually addressable nanopore devices; and
(c) analyzing, with the aid of a processor coupled to said chip, at least a portion of each of said molecules based on electrical signals received from said plurality of individually addressable nanopore devices.

2. The method of claim 1 , each individually addressable nanopore device of said plurality of individually addressable nanopore devices including an electrical circuit including a plurality of components, wherein said plurality of components are controllable by using said row and column addressing.

3. The method of claim 1 , wherein said plurality of molecules comprises a plurality of nucleic acid molecules.

4. The method of claim 1 , wherein said electrode is adapted to supply an electrical stimulus across said membrane, wherein said electrical stimulus enables generation of said electrical signals upon molecular flow of said molecules or portion thereof.

5. The method of claim 1 , wherein each individually addressable nanopore device is adapted to regulate molecular flow with the aid of an electrical stimulus applied to said individually addressable nanopore device.

6. A system for analyzing a plurality of molecules, comprising:
(a) a chip comprising a plurality of individually addressable nanopore devices, each individually addressable nanopore device of said plurality of individually addressable nanopore devices containing at least one nanopore formed in a membrane disposed adjacent to an electrode, each individually addressable nanopore device being addressable using row and column addressing; and
(b) a processor coupled to said chip, wherein said processor is programmed to aid in
analyzing at least a portion of each of said molecules based on electrical signals received from
said plurality of individually addressable nanopore devices.

7. The system of claim 6 , each individually addressable nanopore device of said plurality of individually addressable nanopore devices including an electrical circuit including a plurality of components, wherein said plurality of components are controllable by using said row and column addressing.

8. The system of claim 6 , wherein said plurality of molecules comprises a plurality of nucleic acid molecules.

9. The system of claim 6 , wherein said electrode is adapted to supply an electrical stimulus across said membrane, wherein said electrical stimulus enables generation of said electrical signals upon molecular flow of said molecules or portion thereof.

10. The system of claim 6 , wherein each individually addressable nanopore device is adapted to regulate molecular flow with the aid of an electrical stimulus applied to said individually addressable nanopore device.

Assignees

• Univ California

Inventors

• Akeson Mark A
• Deamer David W
• Dunbar William B
• Chen Roger Jinteh Arrigo
• Wilson Noah A

Classifications

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• C12Q1/6869 Primary

  Methods for sequencing · CPC title

• C25B3/10

  Chemistry & Metallurgy · mapped topic

• C12Q2522/101

  Single or double stranded nucleic acid binding proteins · CPC title

• G01N27/4166

  measuring a particular property of an electrolyte · CPC title