Lung model device for inhalation toxicity testing

Patent metadata

Field	Value
Publication number	US-10338059-B2
Application number	US-201515322894-A
Country	US
Kind code	B2
Filing date	Jun 4, 2015
Priority date	Jul 1, 2014
Publication date	Jul 2, 2019
Grant date	Jul 2, 2019

Abstract

A lung model device for inhalation toxicity testing is provided. The device has a plurality of mesh tissue panels having lung cells attached thereto arranged inside a case so as to have a similar structure to a human lung, and air and nanoparticles are supplied into the case through a separate respiratory operating unit, thereby enabling inhalation toxicity testing on nanoparticles to be simply and conveniently performed in an indirect way by determining changes in the state of the lung cells without using real laboratory animals. The mesh tissue panels having a smaller lattice spacing size are sequentially positioned according to the nanoparticle inflow direction, thereby providing a structure similar to the structure of a real lung.

First claim

The invention claimed is:

1. A lung model device for inhalation toxicity testing, comprising:
a case having an internal space formed therein wherein air and nanoparticles are allowed to flow into and from the internal space;
mesh tissue panels mounted in plural in the internal space of the case, having different lattice spacings, and having lattice lines to which lung cells of a human or an animal are attached,
wherein the plurality of mesh tissue panels are sequentially arranged in the internal space of the case in an inflow direction of the nanoparticles in descending order of sizes of the different lattice spacings thereof.

2. The lung model device of claim 1 , further comprising:
a respiration operating unit allowing the air and the nanoparticles to flow into the internal space of the case by alternately and repeatedly performing an inflow operation and a discharge operation on the air with respect to the internal space of the case; and
a pipe to communicate the respiration operating unit with the internal space,
wherein the respiration operating unit comprises:
a particle supply module allowing nanoparticles and air to flow into the internal space of the case by generating the nanoparticles; and
an air discharge module discharging air from the internal space of the case,
wherein the particle supply module and the air discharge unit alternately and repeatedly operate.

3. The lung model device of claim 2 , wherein the particle supply module comprises:
a particle generator generating nanoparticles; and
an air inflow pump allowing air to flow into the internal space of the case such that the nanoparticles generated by the particle generator flow into the internal space of the case together with the air.

4. The lung model device of claim 3 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

5. The lung model device of claim 4 , wherein the plurality of mesh tissue panels are provided as at least four mesh tissue panels having different lattice spacings, and
a trachea cell, a bronchial tube cell, a bronchial branch cell, and an alveolus cell of the lung cells in the human or the animal are respectively cultivated on the at least four mesh tissue panels having different lattice spacings.

6. The lung model device of claim 2 , wherein
the air discharge module comprises a buffer bag communicating with the internal space of the case such that the air flowing into the internal space of the case through the particle supply module passes through the plurality of mesh tissue panels, and then, flows into the buffer bag,
the buffer bag is formed of an elastic material such that a shape thereof is restored, and
the air flowing into the internal space of the case is discharged from the internal space of the case by elastic restoring force of the buffer bag.

7. The lung model device of claim 6 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

8. The lung model device of claim 7 , wherein the plurality of mesh tissue panels are provided as at least four mesh tissue panels having different lattice spacings, and
a trachea cell, a bronchial tube cell, a bronchial branch cell, and an alveolus cell of the lung cells in the human or the animal are respectively cultivated on the at least four mesh tissue panels having different lattice spacings.

9. The lung model device of claim 2 , wherein the air discharge module comprises:
a buffer bag communicating with the internal space of the case such that the air flowing into the internal space of the case through the particle supply module passes through the plurality of mesh tissue panels, and then, flows into the buffer bag; and
an air discharge pump discharging air from the internal space of the case.

10. The lung model device of claim 9 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

11. The lung model device of claim 10 , wherein the plurality of mesh tissue panels are provided as at least four mesh tissue panels having different lattice spacings, and
a trachea cell, a bronchial tube cell, a bronchial branch cell, and an alveolus cell of the lung cells in the human or the animal are respectively cultivated on the at least four mesh tissue panels having different lattice spacings.

12. The lung model device of claim 2 , wherein:
the pipe includes a main pipe which is mounted in the case to communicate with the internal space such that air flows into and from the internal space, and the main pipe is branched into an inflow pipe and a discharge pipe;
the inflow pipe is connected to the particle supply module such that the air and the nanoparticles flow into the internal space of the case; and
the discharge pipe is formed to have an open end such that air is discharged from the internal space of the case.

13. The lung model device of claim 12 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

14. The lung model device of claim 13 , wherein the plurality of mesh tissue panels are provided as at least four mesh tissue panels having different lattice spacings, and
a trachea cell, a bronchial tube cell, a bronchial branch cell, and an alveolus cell of the lung cells in the human or the animal are respectively cultivated on the at least four mesh tissue panels having different lattice spacings.

15. The lung model device of claim 12 , wherein a channel conversion valve is mounted at a branched portion of the main pipe to selectively open the inflow pipe and the discharge pipe, and operates in a state of being interlocked with an operating state of the particle supply module and an operating state of the air discharge nodule.

16. The lung model device of claim 15 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

17. The lung model device of claim 2 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

18. The lung model device of claim 17 , wherein the plurality of mesh tissue panels are provided as at least four mesh tissue panels having different lattice spacings, and
a trachea cell, a bronchial tube cell, a bronchial branch cell, and an alveolus cell of the lung cells in the human or the animal are respectively cultivated on the at least four mesh tissue panels having different lattice spacings.

19. The lung model device of claim 1 , wherein the plurality of mesh tissue panels are formed such that the lung cells of the human or the animal are uniformly cultivated on the lattice lines of each of the plurality of mesh tissue panels.

20. The lung model device of claim 19 , wherein the plurality of mesh tissue panels are provided as at least four mesh tissue panels having different lattice spacings, and
a trachea cell, a bronchial tube cell, a bronchial branch cell, and an alv

Assignees

• Korea Res Inst Chemical Tech

Inventors

• Lee Kyu Hong
• Yang Hyo Seon

Classifications

• G01N33/5044 Primary

  involving specific cell types · CPC title

• G01N33/5014 Primary

  for testing toxicity · CPC title

• C12M23/00

  Constructional details, e.g. recesses, hinges (flow directing inserts in C12M27/18-C12M27/24; apparatus for chemical or physical processes in general B01J, chemical or physical laboratory apparatus in general B01L) · CPC title

• G01N37/00

  Details not covered by any other group of this subclass · CPC title

• G01N33/48 Primary

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title